Characterization of large deletion mutants of mycobacterium tuberculosis selected for isoniazid resistance

Catherine Vilchèze; Rajagopalan Saranathan; Brian Weinrick; William R. Jacobs

doi:10.1128/AAC.00792-20

Characterization of large deletion mutants of mycobacterium tuberculosis selected for isoniazid resistance

Catherine Vilchèze, Rajagopalan Saranathan, Brian Weinrick, William R. Jacobs

Microbiology & Immunology

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Large genomic deletions (LGDs) (6 to 63 kbp) were observed in isoniazid-resistant Mycobacterium tuberculosis mutants derived from four M. tuberculosis strains. These LGDs had no growth defect in vitro but could be defective in intracellular growth and showed various sensitivities toward oxidative stress despite lacking katG. The LGD regions comprise 74 genes, mostly of unknown function, that may be important for M. tuberculosis intracellular growth and protection against oxidative stress.

Original language	English (US)
Article number	e00792-20
Journal	Antimicrobial agents and chemotherapy
Volume	64
Issue number	9
DOIs	https://doi.org/10.1128/AAC.00792-20
State	Published - Sep 2020

Keywords

Arginine
Deletion
Isoniazid
Resistance

ASJC Scopus subject areas

Pharmacology
Pharmacology (medical)
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1128/AAC.00792-20

Cite this

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abstract = "Large genomic deletions (LGDs) (6 to 63 kbp) were observed in isoniazid-resistant Mycobacterium tuberculosis mutants derived from four M. tuberculosis strains. These LGDs had no growth defect in vitro but could be defective in intracellular growth and showed various sensitivities toward oxidative stress despite lacking katG. The LGD regions comprise 74 genes, mostly of unknown function, that may be important for M. tuberculosis intracellular growth and protection against oxidative stress.",

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AU - Vilchèze, Catherine

AU - Saranathan, Rajagopalan

AU - Weinrick, Brian

AU - Jacobs, William R.

PY - 2020/9

Y1 - 2020/9

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AB - Large genomic deletions (LGDs) (6 to 63 kbp) were observed in isoniazid-resistant Mycobacterium tuberculosis mutants derived from four M. tuberculosis strains. These LGDs had no growth defect in vitro but could be defective in intracellular growth and showed various sensitivities toward oxidative stress despite lacking katG. The LGD regions comprise 74 genes, mostly of unknown function, that may be important for M. tuberculosis intracellular growth and protection against oxidative stress.

KW - Arginine

KW - Deletion

KW - Isoniazid

KW - Resistance

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Characterization of large deletion mutants of mycobacterium tuberculosis selected for isoniazid resistance

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

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