Characterization of Junin arenavirus cell entry

M. Guadalupe Martinez, Sandra M. Cordo, Nélida A. Candurra

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

Junin virus (JUNV) entry is conducted by receptor-mediated endocytosis. To explore the cellular entry mechanism of JUNV, inhibitory effects of drugs affecting the main endocytic pathways on JUNV entry into Vero cells were analysed. Compounds that impair clathrin-mediated endocytosis were shown to reduce virus internalization without affecting virion binding. In contrast, drugs that alter lipid-raft microdomains, impairing caveola-mediated endocytosis, were not able to block virus entry. To show direct evidence of JUNV entry, transmission electron microscopy was performed; it showed JUNV particles of about 60-100 nm in membrane depressions that had an electron-dense coating, In addition, JUNV particles were found within invaginations of the plasma membrane and vesicles that resembled those of pits and clathrin-coated vesicles. Taken together, these results demonstrate that clathrin-mediated endocytosis is the main JUNV entry pathway into Vero cells and represent an important contribution to the characterization of the arenavirus multiplication cycle.

Original languageEnglish (US)
Pages (from-to)1776-1784
Number of pages9
JournalJournal of General Virology
Volume88
Issue number6
DOIs
StatePublished - Jun 2007
Externally publishedYes

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Junin virus
Arenavirus
Virus Internalization
Endocytosis
Virion
Clathrin
Vero Cells
Clathrin-Coated Vesicles
Caveolae
Transmission Electron Microscopy
Pharmaceutical Preparations
Cell Membrane
Electrons
Lipids
Membranes

ASJC Scopus subject areas

  • Virology
  • Immunology

Cite this

Martinez, M. G., Cordo, S. M., & Candurra, N. A. (2007). Characterization of Junin arenavirus cell entry. Journal of General Virology, 88(6), 1776-1784. https://doi.org/10.1099/vir.0.82808-0

Characterization of Junin arenavirus cell entry. / Martinez, M. Guadalupe; Cordo, Sandra M.; Candurra, Nélida A.

In: Journal of General Virology, Vol. 88, No. 6, 06.2007, p. 1776-1784.

Research output: Contribution to journalArticle

Martinez, MG, Cordo, SM & Candurra, NA 2007, 'Characterization of Junin arenavirus cell entry', Journal of General Virology, vol. 88, no. 6, pp. 1776-1784. https://doi.org/10.1099/vir.0.82808-0
Martinez, M. Guadalupe ; Cordo, Sandra M. ; Candurra, Nélida A. / Characterization of Junin arenavirus cell entry. In: Journal of General Virology. 2007 ; Vol. 88, No. 6. pp. 1776-1784.
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