Characterization of hydrogen bonding in the complex of adenosine deaminase with a transition state analogue

A Raman spectroscopic study

Hua Deng, Linda C. Kurz, Frederick B. Rudolph, Robert Callender

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The Raman spectra of purine ribonucleoside as well as a stable model compound (1-methoxyl-1,6-dihydropurine ribonucleoside), free in solution and bound into its complex with adenosine deaminase (ADA), have been studied by Raman difference spectroscopy. Using purine riboside analogues labeled with 15SN1 or 13C6 and the theoretical frequency normal-mode analyses of these molecules using ab initio quantum mechanic methods, we have positively identified many of the Raman bands in the enzymebound inhibitor. The spectrum of the enzyme-bound inhibitor is consistent with the enzyme-catalyzed hydration of the purine base to yield 1-hydroxyl-1,6-dihydropurine ribonucleoside, as suggested earlier by X-ray crystallographic studies. In addition, the Raman data and subsequent vibrational analysis show that the binding-induced Raman spectral changes of the inhibitor can be modeled by the formation of a strong hydrogen bond to its N1-H bond. This hydrogen bond, apparently between the N1-H of the inhibitor and the Oδ1 of Glu217 in ADA, causes a substantial N1-H bending frequency increase of about 50-100 cm-1 compared to its solution value, and this results in an estimated enthalpy of the hydrogen bond of 4-10 kcal/mol. The relationship of transition state stabilization in the catalytic strategy of this efficient enzyme to such a bonding pattern is discussed.

Original languageEnglish (US)
Pages (from-to)4968-4976
Number of pages9
JournalBiochemistry
Volume37
Issue number14
DOIs
StatePublished - Apr 7 1998

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Adenosine Deaminase
Hydrogen Bonding
Ribonucleosides
Hydrogen
Hydrogen bonds
Enzymes
Raman Spectrum Analysis
Quantum theory
Chemical bonds
Enzyme Inhibitors
Hydration
Hydroxyl Radical
Raman scattering
Enthalpy
Stabilization
X-Rays
Spectroscopy
X rays
Molecules
nebularine

ASJC Scopus subject areas

  • Biochemistry

Cite this

Characterization of hydrogen bonding in the complex of adenosine deaminase with a transition state analogue : A Raman spectroscopic study. / Deng, Hua; Kurz, Linda C.; Rudolph, Frederick B.; Callender, Robert.

In: Biochemistry, Vol. 37, No. 14, 07.04.1998, p. 4968-4976.

Research output: Contribution to journalArticle

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