Characterization of human interleukin 1 inhibitor

Z. Liao, A. Haimovitz, Y. Chen, J. Chan, David L. Rosenstreich

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

Human urine contains a specific inhibitor of interleukin 1 (IL 1) that is found in increased amounts during fever. This inhibitor was purified by using a sequence of ammonium sulfate fractionation, DEAE cellulose ion-exchange chromatography, molecular sieve chromatography on Sephacryl S-200, affinity chromatography on concanavalin A (Con A) Sepharose, and polyacrylamide gel electrophoresis. Two peaks of IL 1 inhibitory material were eluted from the polyacrylamide gels. One peak contained three proteins of 29, 32, and 67 kd, respectively, which could be visualized by silver staining. The second peak contained only a small amount of the 67 kd protein. A partially purified inhibitor fraction was found to cross-react with antisera directed against two low m.w. urine trypsin inhibitors that are cleavage products of the serum inter-α-trypsin inhibitor. Although these findings suggest that the urine IL 1 inhibitor may be related to the inter-α-trypsin inhibitor, a more exact identification will require either a homogeneous inhibitor preparation or a monospecific antiserum.

Original languageEnglish (US)
Pages (from-to)3882-3886
Number of pages5
JournalJournal of Immunology
Volume134
Issue number6
StatePublished - 1985
Externally publishedYes

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Trypsin Inhibitors
Interleukin-1
Immune Sera
Urine
Interleukin 1 Receptor Antagonist Protein
DEAE-Cellulose Chromatography
Silver Staining
Ion Exchange Chromatography
Ammonium Sulfate
Affinity Chromatography
Gel Chromatography
Polyacrylamide Gel Electrophoresis
Proteins
Fever
Serum

ASJC Scopus subject areas

  • Immunology

Cite this

Liao, Z., Haimovitz, A., Chen, Y., Chan, J., & Rosenstreich, D. L. (1985). Characterization of human interleukin 1 inhibitor. Journal of Immunology, 134(6), 3882-3886.

Characterization of human interleukin 1 inhibitor. / Liao, Z.; Haimovitz, A.; Chen, Y.; Chan, J.; Rosenstreich, David L.

In: Journal of Immunology, Vol. 134, No. 6, 1985, p. 3882-3886.

Research output: Contribution to journalArticle

Liao, Z, Haimovitz, A, Chen, Y, Chan, J & Rosenstreich, DL 1985, 'Characterization of human interleukin 1 inhibitor', Journal of Immunology, vol. 134, no. 6, pp. 3882-3886.
Liao Z, Haimovitz A, Chen Y, Chan J, Rosenstreich DL. Characterization of human interleukin 1 inhibitor. Journal of Immunology. 1985;134(6):3882-3886.
Liao, Z. ; Haimovitz, A. ; Chen, Y. ; Chan, J. ; Rosenstreich, David L. / Characterization of human interleukin 1 inhibitor. In: Journal of Immunology. 1985 ; Vol. 134, No. 6. pp. 3882-3886.
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