@inbook{eb3aa1315bb94f6c9d505aa66e2ae469,
title = "Characterization of genetically encoded FRET biosensors for Rho-family GTPases",
abstract = "Genetically encoded FRET-based biosensors are increasingly popular and useful tools for examining signaling pathways with high spatial and temporal resolution in living cells. Here, we show basic techniques used to characterize and to validate single-chain, genetically encoded F{\"o}rster resonance energy transfer (FRET) biosensors of the Rho GTPase-family proteins. Methods described here are generally applicable to other genetically encoded FRET-based biosensors by modifying the tested conditions to include additional/different regulators and inhibitors, as appropriate for the specific protein of interest.",
keywords = "Biosensors, FRET, Fluorometry, Ratiometric analysis, Rho GTPases, Validation",
author = "Donnelly, {Sara K.} and Veronika Miskolci and Garrastegui, {Alice M.} and Dianne Cox and Louis Hodgson",
note = "Funding Information: This work was supported by American Cancer Society Lee National Denim Day Postdoctoral Fellowship PF-15-135-01-CSM (S.-D.), Irma T. Hirschl Career Scientist Award (L.H.), and NIH grants: T32GM007491 (V.M.); R01 GM071828 and P01 CA100324 (D.C.); and CA205262 (L.H.). A.M.G. was supported by the Summer Undergraduate Research Program (SURP) of the Albert Einstein College of Medicine, Graduate Division of Biomedical Sciences. Sara K. Donnelly and Veronika Miskolci contributed equally to this work. Publisher Copyright: {\textcopyright} Springer Science+Business Media, LLC, part of Springer Nature 2018.",
year = "2018",
doi = "10.1007/978-1-4939-8612-5_7",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "87--106",
booktitle = "Methods in Molecular Biology",
}