Characterization of gap junctions between cultured leptomeningeal cells

D. C. Spray, A. P. Moreno, J. A. Kessler, R. Dermietzel

Research output: Contribution to journalArticlepeer-review

72 Scopus citations

Abstract

Leptomeningeal cells in intact meninges or dissociated and cultured for 2 h to several weeks were dye-coupled (Lucifer yellow), and voltage-clamped pairs of freshly dissociated leptomeningeal cells were well coupled electrically. Unitary conductances of junctional channels were predominantly 40-90 pS. Junctional conductance was reversibly reduced by 2 mM halothane, 1 mM heptanol and 100% CO2 and was increased by 1 mM 8 Br-cAMP. Two gap junction proteins, connexin 26 and connexin 43, were identified between leptomeningeal cells using immunocytochemical methods; Northern blot analyses of RNA isolated from cultured leptomeningeal cells showed specific hybridization to cDNAs encoding connexins 26 and 43, but not to a cDNA encoding connexin 32. These studies demonstrate co-expression of two connexins in a single cell type in the nervous system; biophysical properties do not differ significantly from those of astrocytes and cardiac myocytes, which express only connexin 43.

Original languageEnglish (US)
Pages (from-to)1-14
Number of pages14
JournalBrain Research
Volume568
Issue number1-2
DOIs
StatePublished - Dec 24 1991

Keywords

  • Connexin 26
  • Connexin 43
  • Cyclic adenosine monophosphate
  • Electronic synapse
  • Halothane
  • Junctional conductance
  • Meninges
  • Messenger ribonucleic acid
  • Single channel

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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