Characterization of endothelial cells using electrochemical impedance spectroscopy

Fei Liu, S. M. Arifuzzaman, A. N. Nordin, David C. Spray, I. Voiculescu

Research output: Chapter in Book/Report/Conference proceedingConference contribution

7 Citations (Scopus)

Abstract

In this paper we report on the electrical impedance spectroscopy characterization of endothelial cell lines (RFPEC). For electrical cell-substrate impedance sensing (ECIS) of the endothelial cells a commercially available eight-well cell culture impedance array (ECIS-8W1E) was used. The impedance measurements were recorded with cell culture medium (without cells) and with endothelial cell layer in the culture medium over a frequency range from 100 Hz to 100 kHz. The impedance measurements were compared to the equivalent circuit model. The impedance measurements of endothelia cells were also simulated using COMSOL Multiphysics™, a commercially available modeling package.

Original languageEnglish (US)
Title of host publicationIEEE Asia-Pacific Conference on Circuits and Systems, Proceedings, APCCAS
Pages252-255
Number of pages4
DOIs
StatePublished - 2010
Event2010 Asia Pacific Conference on Circuit and System, APCCAS 2010 - Kuala Lumpur, Malaysia
Duration: Dec 6 2010Dec 9 2010

Other

Other2010 Asia Pacific Conference on Circuit and System, APCCAS 2010
CountryMalaysia
CityKuala Lumpur
Period12/6/1012/9/10

Fingerprint

Endothelial cells
Electrochemical impedance spectroscopy
Cell culture
Acoustic impedance
Substrates
Equivalent circuits
Spectroscopy

Keywords

  • cell-substrate impedance sensing (ECIS)
  • COMSOL Multiphysics
  • eight-well cell culture impedance array
  • impedance
  • reactance

ASJC Scopus subject areas

  • Electrical and Electronic Engineering

Cite this

Liu, F., Arifuzzaman, S. M., Nordin, A. N., Spray, D. C., & Voiculescu, I. (2010). Characterization of endothelial cells using electrochemical impedance spectroscopy. In IEEE Asia-Pacific Conference on Circuits and Systems, Proceedings, APCCAS (pp. 252-255). [5774824] https://doi.org/10.1109/APCCAS.2010.5774824

Characterization of endothelial cells using electrochemical impedance spectroscopy. / Liu, Fei; Arifuzzaman, S. M.; Nordin, A. N.; Spray, David C.; Voiculescu, I.

IEEE Asia-Pacific Conference on Circuits and Systems, Proceedings, APCCAS. 2010. p. 252-255 5774824.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Liu, F, Arifuzzaman, SM, Nordin, AN, Spray, DC & Voiculescu, I 2010, Characterization of endothelial cells using electrochemical impedance spectroscopy. in IEEE Asia-Pacific Conference on Circuits and Systems, Proceedings, APCCAS., 5774824, pp. 252-255, 2010 Asia Pacific Conference on Circuit and System, APCCAS 2010, Kuala Lumpur, Malaysia, 12/6/10. https://doi.org/10.1109/APCCAS.2010.5774824
Liu F, Arifuzzaman SM, Nordin AN, Spray DC, Voiculescu I. Characterization of endothelial cells using electrochemical impedance spectroscopy. In IEEE Asia-Pacific Conference on Circuits and Systems, Proceedings, APCCAS. 2010. p. 252-255. 5774824 https://doi.org/10.1109/APCCAS.2010.5774824
Liu, Fei ; Arifuzzaman, S. M. ; Nordin, A. N. ; Spray, David C. ; Voiculescu, I. / Characterization of endothelial cells using electrochemical impedance spectroscopy. IEEE Asia-Pacific Conference on Circuits and Systems, Proceedings, APCCAS. 2010. pp. 252-255
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