Characterization of endothelial cells using electrochemical impedance spectroscopy

Fei Liu, S. M. Arifuzzaman, A. N. Nordin, D. Spray, I. Voiculescu

Research output: Chapter in Book/Report/Conference proceedingConference contribution

8 Scopus citations

Abstract

In this paper we report on the electrical impedance spectroscopy characterization of endothelial cell lines (RFPEC). For electrical cell-substrate impedance sensing (ECIS) of the endothelial cells a commercially available eight-well cell culture impedance array (ECIS-8W1E) was used. The impedance measurements were recorded with cell culture medium (without cells) and with endothelial cell layer in the culture medium over a frequency range from 100 Hz to 100 kHz. The impedance measurements were compared to the equivalent circuit model. The impedance measurements of endothelia cells were also simulated using COMSOL Multiphysics™, a commercially available modeling package.

Original languageEnglish (US)
Title of host publicationProceedings of the 2010 Asia Pacific Conference on Circuit and System, APCCAS 2010
Pages252-255
Number of pages4
DOIs
StatePublished - Dec 1 2010
Event2010 Asia Pacific Conference on Circuit and System, APCCAS 2010 - Kuala Lumpur, Malaysia
Duration: Dec 6 2010Dec 9 2010

Publication series

NameIEEE Asia-Pacific Conference on Circuits and Systems, Proceedings, APCCAS

Other

Other2010 Asia Pacific Conference on Circuit and System, APCCAS 2010
CountryMalaysia
CityKuala Lumpur
Period12/6/1012/9/10

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Keywords

  • COMSOL Multiphysics
  • cell-substrate impedance sensing (ECIS)
  • eight-well cell culture impedance array
  • impedance
  • reactance

ASJC Scopus subject areas

  • Electrical and Electronic Engineering

Cite this

Liu, F., Arifuzzaman, S. M., Nordin, A. N., Spray, D., & Voiculescu, I. (2010). Characterization of endothelial cells using electrochemical impedance spectroscopy. In Proceedings of the 2010 Asia Pacific Conference on Circuit and System, APCCAS 2010 (pp. 252-255). [5774824] (IEEE Asia-Pacific Conference on Circuits and Systems, Proceedings, APCCAS). https://doi.org/10.1109/APCCAS.2010.5774824