Characterization of angiotensin-(1-7) receptor subtype in mesenteric arteries

Liomar A.A. Neves, David B. Averill, Carlos M. Ferrario, Mark C. Chappell, Judy L. Aschner, Michael P. Walkup, K. Bridget Brosnihan

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Abstract

Mesenteric arteries from male Sprague-Dawley rats were mounted in a pressurized myograph system. Ang-(1-7) concentration-dependent responses were determined in arteries preconstricted with endothelin-1 (10-7M). The receptor(s) mediating the Ang-(1-7) evoked dilation were investigated by pretreating the mesenteric arteries with specific antagonists of Ang-(1-7), AT1 or AT2 receptors. The effects of Ang-(3-8) and Ang-(3-7) were also determined. Ang-(1-7) caused a concentration-dependent dilation (EC50: 0.95nM) that was blocked by the selective Ang-(1-7) receptor antagonist D-[Ala7]-Ang-(1-7). Administration of a specific antagonist to the AT2 receptor (PD123319) had no effect. On the other hand, losartan and CV-11974 attenuated the Ang-(1-7) effect. These results demonstrate that Ang-(1-7) elicits potent dilation of mesenteric resistance vessels mediated by a D-[Ala7]-Ang-(1-7) sensitive site that is also sensitive to losartan and CV-11974.

Original languageEnglish (US)
Pages (from-to)455-462
Number of pages8
JournalPeptides
Volume24
Issue number3
DOIs
Publication statusPublished - Mar 1 2003

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Keywords

  • Angiotensin receptors
  • Hypertension
  • Renin-angiotensin system
  • Vasodilation

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Endocrinology
  • Cellular and Molecular Neuroscience

Cite this

Neves, L. A. A., Averill, D. B., Ferrario, C. M., Chappell, M. C., Aschner, J. L., Walkup, M. P., & Brosnihan, K. B. (2003). Characterization of angiotensin-(1-7) receptor subtype in mesenteric arteries. Peptides, 24(3), 455-462. https://doi.org/10.1016/S0196-9781(03)00062-7