Characterization of angiotensin-(1-7) receptor subtype in mesenteric arteries

Liomar A.A. Neves; David B. Averill; Carlos M. Ferrario; Mark C. Chappell; Judy L. Aschner; Michael P. Walkup; K. Bridget Brosnihan

doi:10.1016/S0196-9781(03)00062-7

Characterization of angiotensin-(1-7) receptor subtype in mesenteric arteries

Liomar A.A. Neves, David B. Averill, Carlos M. Ferrario, Mark C. Chappell, Judy L. Aschner, Michael P. Walkup, K. Bridget Brosnihan

Research output: Contribution to journal › Article › peer-review

30 Scopus citations

Abstract

Mesenteric arteries from male Sprague-Dawley rats were mounted in a pressurized myograph system. Ang-(1-7) concentration-dependent responses were determined in arteries preconstricted with endothelin-1 (10^-7M). The receptor(s) mediating the Ang-(1-7) evoked dilation were investigated by pretreating the mesenteric arteries with specific antagonists of Ang-(1-7), AT₁ or AT₂ receptors. The effects of Ang-(3-8) and Ang-(3-7) were also determined. Ang-(1-7) caused a concentration-dependent dilation (EC₅₀: 0.95nM) that was blocked by the selective Ang-(1-7) receptor antagonist D-[Ala⁷]-Ang-(1-7). Administration of a specific antagonist to the AT₂ receptor (PD123319) had no effect. On the other hand, losartan and CV-11974 attenuated the Ang-(1-7) effect. These results demonstrate that Ang-(1-7) elicits potent dilation of mesenteric resistance vessels mediated by a D-[Ala⁷]-Ang-(1-7) sensitive site that is also sensitive to losartan and CV-11974.

Original language	English (US)
Pages (from-to)	455-462
Number of pages	8
Journal	Peptides
Volume	24
Issue number	3
DOIs	https://doi.org/10.1016/S0196-9781(03)00062-7
State	Published - Mar 1 2003
Externally published	Yes

Keywords

Angiotensin receptors
Hypertension
Renin-angiotensin system
Vasodilation

ASJC Scopus subject areas

Biochemistry
Physiology
Endocrinology
Cellular and Molecular Neuroscience

Access to Document

10.1016/S0196-9781(03)00062-7

Cite this

@article{46e2415ff40946859e646be51a92331a,

title = "Characterization of angiotensin-(1-7) receptor subtype in mesenteric arteries",

abstract = "Mesenteric arteries from male Sprague-Dawley rats were mounted in a pressurized myograph system. Ang-(1-7) concentration-dependent responses were determined in arteries preconstricted with endothelin-1 (10-7M). The receptor(s) mediating the Ang-(1-7) evoked dilation were investigated by pretreating the mesenteric arteries with specific antagonists of Ang-(1-7), AT1 or AT2 receptors. The effects of Ang-(3-8) and Ang-(3-7) were also determined. Ang-(1-7) caused a concentration-dependent dilation (EC50: 0.95nM) that was blocked by the selective Ang-(1-7) receptor antagonist D-[Ala7]-Ang-(1-7). Administration of a specific antagonist to the AT2 receptor (PD123319) had no effect. On the other hand, losartan and CV-11974 attenuated the Ang-(1-7) effect. These results demonstrate that Ang-(1-7) elicits potent dilation of mesenteric resistance vessels mediated by a D-[Ala7]-Ang-(1-7) sensitive site that is also sensitive to losartan and CV-11974.",

keywords = "Angiotensin receptors, Hypertension, Renin-angiotensin system, Vasodilation",

author = "Neves, {Liomar A.A.} and Averill, {David B.} and Ferrario, {Carlos M.} and Chappell, {Mark C.} and Aschner, {Judy L.} and Walkup, {Michael P.} and Brosnihan, {K. Bridget}",

note = "Funding Information: The authors gratefully acknowledge the technical support of Thuy Smith. This work was supported in part by a grant from the National Institutes of Health, NHLBI-P01 HL58952, and HL62489.",

year = "2003",

month = mar,

day = "1",

doi = "10.1016/S0196-9781(03)00062-7",

language = "English (US)",

volume = "24",

pages = "455--462",

journal = "Peptides",

issn = "0196-9781",

publisher = "Elsevier Inc.",

number = "3",

}

TY - JOUR

T1 - Characterization of angiotensin-(1-7) receptor subtype in mesenteric arteries

AU - Neves, Liomar A.A.

AU - Averill, David B.

AU - Ferrario, Carlos M.

AU - Chappell, Mark C.

AU - Aschner, Judy L.

AU - Walkup, Michael P.

AU - Brosnihan, K. Bridget

N1 - Funding Information: The authors gratefully acknowledge the technical support of Thuy Smith. This work was supported in part by a grant from the National Institutes of Health, NHLBI-P01 HL58952, and HL62489.

PY - 2003/3/1

Y1 - 2003/3/1

N2 - Mesenteric arteries from male Sprague-Dawley rats were mounted in a pressurized myograph system. Ang-(1-7) concentration-dependent responses were determined in arteries preconstricted with endothelin-1 (10-7M). The receptor(s) mediating the Ang-(1-7) evoked dilation were investigated by pretreating the mesenteric arteries with specific antagonists of Ang-(1-7), AT1 or AT2 receptors. The effects of Ang-(3-8) and Ang-(3-7) were also determined. Ang-(1-7) caused a concentration-dependent dilation (EC50: 0.95nM) that was blocked by the selective Ang-(1-7) receptor antagonist D-[Ala7]-Ang-(1-7). Administration of a specific antagonist to the AT2 receptor (PD123319) had no effect. On the other hand, losartan and CV-11974 attenuated the Ang-(1-7) effect. These results demonstrate that Ang-(1-7) elicits potent dilation of mesenteric resistance vessels mediated by a D-[Ala7]-Ang-(1-7) sensitive site that is also sensitive to losartan and CV-11974.

AB - Mesenteric arteries from male Sprague-Dawley rats were mounted in a pressurized myograph system. Ang-(1-7) concentration-dependent responses were determined in arteries preconstricted with endothelin-1 (10-7M). The receptor(s) mediating the Ang-(1-7) evoked dilation were investigated by pretreating the mesenteric arteries with specific antagonists of Ang-(1-7), AT1 or AT2 receptors. The effects of Ang-(3-8) and Ang-(3-7) were also determined. Ang-(1-7) caused a concentration-dependent dilation (EC50: 0.95nM) that was blocked by the selective Ang-(1-7) receptor antagonist D-[Ala7]-Ang-(1-7). Administration of a specific antagonist to the AT2 receptor (PD123319) had no effect. On the other hand, losartan and CV-11974 attenuated the Ang-(1-7) effect. These results demonstrate that Ang-(1-7) elicits potent dilation of mesenteric resistance vessels mediated by a D-[Ala7]-Ang-(1-7) sensitive site that is also sensitive to losartan and CV-11974.

KW - Angiotensin receptors

KW - Hypertension

KW - Renin-angiotensin system

KW - Vasodilation

UR - http://www.scopus.com/inward/record.url?scp=0038066401&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0038066401&partnerID=8YFLogxK

U2 - 10.1016/S0196-9781(03)00062-7

DO - 10.1016/S0196-9781(03)00062-7

M3 - Article

C2 - 12732345

AN - SCOPUS:0038066401

SN - 0196-9781

VL - 24

SP - 455

EP - 462

JO - Peptides

JF - Peptides

IS - 3

ER -

Characterization of angiotensin-(1-7) receptor subtype in mesenteric arteries

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this