Characterization of an acyclovir-resistant herpes simplex virus type 2 strain isolated from a premature neonate

Ronda J. Oram, Daniel Marcellino, Daniel Strauss, Erik Gustafson, Christine L. Talarico, Adrienne K. Root, Prem L. Sharma, Ken Thompson, Joyce D. Fingeroth, Clyde Crumpacker, Betsy C. Herold

Research output: Contribution to journalArticle

48 Scopus citations

Abstract

Acyclovir resistance is not a recognized problem among neonates with perinatal herpes simplex virus (HSV) infection. A premature newborn with neurocutaneous HSV infection was treated for 21 days with acyclovir. Disseminated disease recurred 8 days later. A recurrent isolate was resistant to acyclovir and lacked thymidine kinase activity on the basis of a frame- shift mutation in the thymidine kinase (tk) gene. Compared with the sensitive isolate obtained during primary infection, replication of the resistant isolate was reduced on primary and permanent cells and even further impaired on cells deleted for cellular tk. The resistant isolate lacked virulence in a murine model of genital infection. Acyclovir-resistant HSV-2 mutants can develop rapidly in neonatal infection and cause clinically significant disease, despite decreased replication in vitro and attenuated virulence in an animal model.

Original languageEnglish (US)
Pages (from-to)1458-1461
Number of pages4
JournalJournal of Infectious Diseases
Volume181
Issue number4
DOIs
StatePublished - May 22 2000

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

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    Oram, R. J., Marcellino, D., Strauss, D., Gustafson, E., Talarico, C. L., Root, A. K., Sharma, P. L., Thompson, K., Fingeroth, J. D., Crumpacker, C., & Herold, B. C. (2000). Characterization of an acyclovir-resistant herpes simplex virus type 2 strain isolated from a premature neonate. Journal of Infectious Diseases, 181(4), 1458-1461. https://doi.org/10.1086/315387