Characterization of adipose-derived mesenchymal stem cell combinations for vascularized bone engineering

Cristian D. Valenzuela, Alexander C. Allori, Derek D. Reformat, Alexander M. Sailon, Robert J. Allen, Edward H. Davidson, Mani Alikhani, Timothy G. Bromage, John L. Ricci, Stephen M. Warren

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Since bone repair and regeneration depend on vasculogenesis and osteogenesis, both of these processes are essential for successful vascularized bone engineering. Using adipose-derived stem cells (ASCs), we investigated temporal gene expression profiles, as well as bone nodule and endothelial tubule formation capacities, during osteogenic and vasculogenic ASC lineage commitment. Osteoprogenitor-enriched cell populations were found to express RUNX2, MSX2, SP7 (osterix), BGLAP (osteocalcin), SPARC (osteonectin), and SPP1 (osteopontin) in a temporally specific sequence. Irreversible commitment of ASCs to the osteogenic lineage occurred between days 6 and 9 of differentiation. Endothelioprogenitor-enriched cell populations expressed CD34, PECAM1 (CD31), ENG (CD105), FLT1 (Vascular endothelial growth factor [VEGFR1]), and KDR (VEGFR2). Capacity for microtubule formation was evident in as early as 3 days. Functional capacity was assessed in eight coculture combinations for both bone nodule and endothelial tubule formation, and the greatest expression of these end-differentiation phenotypes was observed in the combination of well-differentiated endothelial cells with less-differentiated osteoblastic cells. Taken together, our results demonstrate vascularized bone engineering utilizing ASCs is a promising enterprise, and that coculture strategies should focus on developing a more mature vascular network in combination with a less mature osteoblastic stromal cell.

Original languageEnglish (US)
Pages (from-to)1373-1385
Number of pages13
JournalTissue Engineering - Part A
Volume19
Issue number11-12
DOIs
StatePublished - Jun 1 2013
Externally publishedYes

Fingerprint

Stem cells
Mesenchymal Stromal Cells
Bone
Stem Cells
Bone and Bones
Coculture Techniques
Osteonectin
Bone Regeneration
Cells
Osteocalcin
Cell Lineage
Stromal Cells
Transcriptome
Osteogenesis
Microtubules
Vascular Endothelial Growth Factor A
Population
Endothelial cells
Blood Vessels
Endothelial Cells

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Biomaterials
  • Biomedical Engineering

Cite this

Valenzuela, C. D., Allori, A. C., Reformat, D. D., Sailon, A. M., Allen, R. J., Davidson, E. H., ... Warren, S. M. (2013). Characterization of adipose-derived mesenchymal stem cell combinations for vascularized bone engineering. Tissue Engineering - Part A, 19(11-12), 1373-1385. https://doi.org/10.1089/ten.tea.2012.0323

Characterization of adipose-derived mesenchymal stem cell combinations for vascularized bone engineering. / Valenzuela, Cristian D.; Allori, Alexander C.; Reformat, Derek D.; Sailon, Alexander M.; Allen, Robert J.; Davidson, Edward H.; Alikhani, Mani; Bromage, Timothy G.; Ricci, John L.; Warren, Stephen M.

In: Tissue Engineering - Part A, Vol. 19, No. 11-12, 01.06.2013, p. 1373-1385.

Research output: Contribution to journalArticle

Valenzuela, CD, Allori, AC, Reformat, DD, Sailon, AM, Allen, RJ, Davidson, EH, Alikhani, M, Bromage, TG, Ricci, JL & Warren, SM 2013, 'Characterization of adipose-derived mesenchymal stem cell combinations for vascularized bone engineering', Tissue Engineering - Part A, vol. 19, no. 11-12, pp. 1373-1385. https://doi.org/10.1089/ten.tea.2012.0323
Valenzuela, Cristian D. ; Allori, Alexander C. ; Reformat, Derek D. ; Sailon, Alexander M. ; Allen, Robert J. ; Davidson, Edward H. ; Alikhani, Mani ; Bromage, Timothy G. ; Ricci, John L. ; Warren, Stephen M. / Characterization of adipose-derived mesenchymal stem cell combinations for vascularized bone engineering. In: Tissue Engineering - Part A. 2013 ; Vol. 19, No. 11-12. pp. 1373-1385.
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