Characterization of acute brain injuries and neurobehavioral profiles in a rabbit model of germinal matrix hemorrhage

Paraskevi Georgiadis, Hongmin Xu, Caroline Chua, Furong Hu, Lee Collins, Chau Huynh, Edmund F. Lagamma, Praveen Ballabh

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

Background and Purpose: Germinal matrix hemorrhage-intraventricular hemorrhage (GMH-IVH) is the most common neurological problem of premature infants and has enormous financial and social impact. Despite this, there is no standardized animal model of IVH depicting acute brain injuries. Methods: We delivered rabbit-pups prematurely at 29-day gestation by C-section, administered intraperitoneal glycerol to the pups at 3-hour postnatal age to induce IVH, and evaluated the brain for evidence of injuries. Results: About 80% of glycerol-treated pups developed gross IVH. We found greater neutrophil and microglia infiltration around the ventricles (periventricular zone) in pups with IVH than in controls. We noted more apoptosis and neuronal degeneration in the periventricular zone than in the neocortex in pups with IVH, but not in controls. There was evidence of axonal damage revealed by β-amyloid precursor protein and neurofilament immunolabeling. Neurobehavioral testing showed that pups with IVH were more wobbly with lesser capability to walk on inclination than pups without IVH. There was no evidence of acute systemic toxicity in the glycerol-treated pups. An evaluation of autopsy materials from premature infants revealed similar evidence of apoptosis and cellular infiltration in the periventricular zone in cases with IVH, but not in cases without IVH-suggesting clinical relevance of the model. Conclusion: The study provides an instructive animal model of IVH with evidence of acute brain injuries that can be used to evaluate strategies in prevention of IVH and acute posthemorrhagic complications.

Original languageEnglish (US)
Pages (from-to)3378-3388
Number of pages11
JournalStroke
Volume39
Issue number12
DOIs
StatePublished - Dec 1 2008
Externally publishedYes

Fingerprint

Glycerol
Brain Injuries
Hemorrhage
Rabbits
Premature Infants
Animal Models
Apoptosis
Intermediate Filaments
Neutrophil Infiltration
Amyloid beta-Protein Precursor
Neocortex
Microglia
Social Change
Autopsy
Pregnancy

Keywords

  • Apoptosis
  • Axonal damage
  • GM hemorrhage-intraventricular hemorrhage
  • Inflammation
  • Microglia
  • Neurobehavioral testing
  • Neutrophil

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Clinical Neurology
  • Advanced and Specialized Nursing
  • Medicine(all)

Cite this

Characterization of acute brain injuries and neurobehavioral profiles in a rabbit model of germinal matrix hemorrhage. / Georgiadis, Paraskevi; Xu, Hongmin; Chua, Caroline; Hu, Furong; Collins, Lee; Huynh, Chau; Lagamma, Edmund F.; Ballabh, Praveen.

In: Stroke, Vol. 39, No. 12, 01.12.2008, p. 3378-3388.

Research output: Contribution to journalArticle

Georgiadis, Paraskevi ; Xu, Hongmin ; Chua, Caroline ; Hu, Furong ; Collins, Lee ; Huynh, Chau ; Lagamma, Edmund F. ; Ballabh, Praveen. / Characterization of acute brain injuries and neurobehavioral profiles in a rabbit model of germinal matrix hemorrhage. In: Stroke. 2008 ; Vol. 39, No. 12. pp. 3378-3388.
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