Characterization of a T4+/Leu-8+ T cell clone that directly helps B cell Ig production by secreting B cell differentiation factor

Harris Goldstein, D. J. Volkman, J. L. Ambrus, A. S. Fauci

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

A human T4+/Leu-8+ T cell clone (YA2) was established by phytohemagglutinin activation and interleukin 2 (IL 2) propagation. Functional characterization of this clone demonstrated that it provided potent help towards Ig production by pokeweed mitogen-stimulated B cells in the presence of small numbers of autologous T cells or by Staphylococcus aureus Cowan I (SAC)-activated B cells in the presence of B cell growth factor (BCGF). YA2 provided no help to resting B cells and minimal help to either unactivated B cells cultured with BCGF or SAC-activated B cells. Supernatant generated from clone YA2 by IL 2 stimlation had significant B cell differentiation activity but no BCGF or IL 2 activity. Thus, YA2 is a T4+/Leu-8+ potent direct helper only to B cells that are activated and proliferating due to its selective secretion of a differentiation factor, and not an activation and growth factor. The availability of phenotypically defined cloned populations of T cells with restricted functional helper activity related to the secretion of selected B cell tropic factors should prove useful in the dissection of the role of individual T cell subsets in the regulation of the human B cell cycle.

Original languageEnglish (US)
Pages (from-to)339-343
Number of pages5
JournalJournal of Immunology
Volume135
Issue number1
StatePublished - 1985
Externally publishedYes

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Interleukin-6
B-Lymphocytes
Clone Cells
T-Lymphocytes
Intercellular Signaling Peptides and Proteins
Interleukin-2
Staphylococcus aureus
Pokeweed Mitogens
T-Lymphocyte Subsets
Phytohemagglutinins
Dissection
Cell Differentiation
Cell Cycle

ASJC Scopus subject areas

  • Immunology

Cite this

Characterization of a T4+/Leu-8+ T cell clone that directly helps B cell Ig production by secreting B cell differentiation factor. / Goldstein, Harris; Volkman, D. J.; Ambrus, J. L.; Fauci, A. S.

In: Journal of Immunology, Vol. 135, No. 1, 1985, p. 339-343.

Research output: Contribution to journalArticle

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