Characterization of a novel mRNA expressed by neurons in mature brain

J. A. Cohen, M. Arai, E. Luning Prak, S. A. Brooks, L. H. Young, Michael B. Prystowsky

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

In previous studies, differential hybridization screening of an activated murine T-lymphocyte cDNA library identified an interleukin 2-responsive mRNA, designated F5, expressed in lymphoid tissues and brain only. We now report characterization of a full-length clone isolated from an adult mouse brain cDNA library. Neither the nucleic acid nor amino acid sequences demonstrated similarity to reported sequences. On Southern blotting, the protein coding sequence hybridized to genomic DNA from a variety of species. On Northern blotting, F5 mRNA was expressed in adult mouse brain, spinal cord, eye, and dorsal root ganglia but not in peripheral nerve. In situ hybridization studies demonstrated prominent expression by neurons in brain. F5 mRNA expression was undetectable in embryonic rat cerebral hemisphere and low until postnatal day 21. F5 is a novel mRNA selectively expressed by proliferating lymphocytes and mature neurons.

Original languageEnglish (US)
Pages (from-to)273-284
Number of pages12
JournalJournal of Neuroscience Research
Volume31
Issue number2
DOIs
StatePublished - 1992
Externally publishedYes

Fingerprint

Neurons
Messenger RNA
Brain
Gene Library
Cerebrum
Spinal Ganglia
Lymphoid Tissue
Southern Blotting
Peripheral Nerves
Northern Blotting
Nucleic Acids
Interleukin-2
In Situ Hybridization
Amino Acid Sequence
Spinal Cord
Clone Cells
Lymphocytes
T-Lymphocytes
DNA
Proteins

Keywords

  • brain development
  • cDNA cloning
  • growth factors
  • interleukin 2
  • lymphocyte activation

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Characterization of a novel mRNA expressed by neurons in mature brain. / Cohen, J. A.; Arai, M.; Luning Prak, E.; Brooks, S. A.; Young, L. H.; Prystowsky, Michael B.

In: Journal of Neuroscience Research, Vol. 31, No. 2, 1992, p. 273-284.

Research output: Contribution to journalArticle

Cohen, J. A. ; Arai, M. ; Luning Prak, E. ; Brooks, S. A. ; Young, L. H. ; Prystowsky, Michael B. / Characterization of a novel mRNA expressed by neurons in mature brain. In: Journal of Neuroscience Research. 1992 ; Vol. 31, No. 2. pp. 273-284.
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