Characterization of a novel intracellular sphingolipid-gated Ca2+-permeable channel from rat basophilic leukemia cells

L. Allen Kindman, Stella Kim, Thomas V. McDonald, Phyllis Gardner

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

Sphingolipids stimulate the release of Ca2+ from intracellular stores. However, the mechanism by which this process occurs has not been characterized. Through single-channel recording from microsomes incorporated into planar lipid bilayers, we describe a novel channel that gates Ba2+ in response to sphingosylphosphorylcholine. The channel is both ligand-gated and voltage-modulated. Maximal open probability is observed between -10 and -20 mV and has a relatively high conductance (160 picosiemens with 53 mM Ba2+). We also observe that Ca2+ efflux from permeabilized rat basophilic leukemia cells is not antagonized by heparin, La3+, Ni2+, nifedipine, or ω-conotoxin GVIa. The sphingolipid-gated Ca2+-permeable channel is therefore a new member of the Ca2+-permeable, ligand-gated channel family.

Original languageEnglish (US)
Pages (from-to)13088-13091
Number of pages4
JournalJournal of Biological Chemistry
Volume269
Issue number18
StatePublished - May 6 1994
Externally publishedYes

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Sphingolipids
Rats
Leukemia
Conotoxins
Ligand-Gated Ion Channels
Lipid bilayers
Lipid Bilayers
Nifedipine
Microsomes
Heparin
Ligands
Electric potential
sphingosine phosphorylcholine

ASJC Scopus subject areas

  • Biochemistry

Cite this

Characterization of a novel intracellular sphingolipid-gated Ca2+-permeable channel from rat basophilic leukemia cells. / Kindman, L. Allen; Kim, Stella; McDonald, Thomas V.; Gardner, Phyllis.

In: Journal of Biological Chemistry, Vol. 269, No. 18, 06.05.1994, p. 13088-13091.

Research output: Contribution to journalArticle

Kindman, L. Allen ; Kim, Stella ; McDonald, Thomas V. ; Gardner, Phyllis. / Characterization of a novel intracellular sphingolipid-gated Ca2+-permeable channel from rat basophilic leukemia cells. In: Journal of Biological Chemistry. 1994 ; Vol. 269, No. 18. pp. 13088-13091.
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