Characterization of a novel intracellular sphingolipid-gated Ca2+- permeable channel from rat basophilic leukemia cells

L. A. Kindman, S. Kim, T. V. McDonald, P. Gardner

Research output: Contribution to journalArticle

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Abstract

Sphingolipids stimulate the release of Ca2+ from intracellular stores. However, the mechanism by which this process occurs has not been characterized. Through single-channel recording from microsomes incorporated into planar lipid bilayers, we describe a novel channel that gates Ba2+ in response to sphingosylphosphorylcholine. The channel is both ligand-gated and voltage-modulated. Maximal open probability is observed between -10 and -20 mV and has a relatively high conductance (160 picosiemens with 53 mM Ba2+). We also observe that Ca2+ efflux from permeabilized rat basophilic leukemia cells is not antagonized by heparin, La3+, Ni2+, nifedipine, or ω- conotoxin GVIa. The sphingolipid-gated Ca2+-permeable channel is therefore a new member of the Ca2+-permeable, ligand-gated channel family.

Original languageEnglish (US)
Pages (from-to)13088-13091
Number of pages4
JournalJournal of Biological Chemistry
Volume269
Issue number18
Publication statusPublished - Jul 6 1994

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ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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