Characterization of a novel 66 kd subunit of mammalian neurofilaments

F. C. Chiu, E. A. Barnes, K. Das, J. Haley, P. Socolow, Frank P. Macaluso, J. Fant

Research output: Contribution to journalArticle

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Abstract

A 66 kd protein, pi 5.4, was purified from the Triton-insoluble fraction of rat spinal cord. This protein formed 10 nm filaments in vitro. The 66 kd protein was unique, although it shared homology with the 70 kd neurofilament protein (NF-L) and vimentin. An antiserum (anti-66) specific to the 66 kd protein did not cross-react with any of the neurofilament triplet proteins. In the spinal cord, anti-66 intensely stained the axons of the anterior and lateral columns. However, afferents from dorsal root ganglia and the efferents from the motoneurons were negative. In the cerebellum, anti-66 intensely stained most axons. The 66 kd protein was readily detectable in homogenates of forebrain, cerebellum, brainstem, and spinal cord, but was found only in trace amounts in adult sciatic nerves and was not found in extraneural tissues. The 66 kd protein constituted 0.5% of total protein in the spinal cord, whereas NF-L constituted about 1.5%.

Original languageEnglish (US)
Pages (from-to)1435-1445
Number of pages11
JournalNeuron
Volume2
Issue number5
DOIs
StatePublished - 1989

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Intermediate Filaments
Neurofilament Proteins
Spinal Cord
Proteins
Cerebellum
Axons
Spinal Ganglia
Motor Neurons
Vimentin
Sciatic Nerve
Prosencephalon
Brain Stem
Immune Sera

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Chiu, F. C., Barnes, E. A., Das, K., Haley, J., Socolow, P., Macaluso, F. P., & Fant, J. (1989). Characterization of a novel 66 kd subunit of mammalian neurofilaments. Neuron, 2(5), 1435-1445. https://doi.org/10.1016/0896-6273(89)90189-X

Characterization of a novel 66 kd subunit of mammalian neurofilaments. / Chiu, F. C.; Barnes, E. A.; Das, K.; Haley, J.; Socolow, P.; Macaluso, Frank P.; Fant, J.

In: Neuron, Vol. 2, No. 5, 1989, p. 1435-1445.

Research output: Contribution to journalArticle

Chiu, FC, Barnes, EA, Das, K, Haley, J, Socolow, P, Macaluso, FP & Fant, J 1989, 'Characterization of a novel 66 kd subunit of mammalian neurofilaments', Neuron, vol. 2, no. 5, pp. 1435-1445. https://doi.org/10.1016/0896-6273(89)90189-X
Chiu, F. C. ; Barnes, E. A. ; Das, K. ; Haley, J. ; Socolow, P. ; Macaluso, Frank P. ; Fant, J. / Characterization of a novel 66 kd subunit of mammalian neurofilaments. In: Neuron. 1989 ; Vol. 2, No. 5. pp. 1435-1445.
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abstract = "A 66 kd protein, pi 5.4, was purified from the Triton-insoluble fraction of rat spinal cord. This protein formed 10 nm filaments in vitro. The 66 kd protein was unique, although it shared homology with the 70 kd neurofilament protein (NF-L) and vimentin. An antiserum (anti-66) specific to the 66 kd protein did not cross-react with any of the neurofilament triplet proteins. In the spinal cord, anti-66 intensely stained the axons of the anterior and lateral columns. However, afferents from dorsal root ganglia and the efferents from the motoneurons were negative. In the cerebellum, anti-66 intensely stained most axons. The 66 kd protein was readily detectable in homogenates of forebrain, cerebellum, brainstem, and spinal cord, but was found only in trace amounts in adult sciatic nerves and was not found in extraneural tissues. The 66 kd protein constituted 0.5{\%} of total protein in the spinal cord, whereas NF-L constituted about 1.5{\%}.",
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