Characterization of a human βv-tubulin antibody and expression of this isotype in normal and malignant human tissue

Suzan K. Chao, Yihong Wang, Pascal Verdier-Pinard, Chia-Ping H. Yang, Lingling Liu, Alicia Rodriguez-Gabin, Hayley M. McDaid, Susan Band Horwitz

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

There are seven distinct β-tubulin isotypes and eight α-tubulin isotypes in mammals that are hypothesized to have tissue- and cell-specific functions. There is an interest in the use of tubulin isotypes as prognostic markers of malignancy. βV-tubulin, like βIII-tubulin, has been implicated in malignant transformation and drug resistance, however little is known about its localization and function. Thus, we generated for the first time, a rabbit polyclonal antibody specific for human βV-tubulin. The antibody did not cross-react with mouse βV-tubulin or other human β-tubulin isotypes and specifically labeled βV-tubulin by immunoblotting, immunofluorescence and immunohistochemistry. Immunohistochemistry of various human normal tissues revealed that βV-tubulin was expressed in endothelial cells, myocytes and cells with muscle differentiation, structures with transport and/or secretory function such as renal tubules, pancreatic ducts and bile ducts, and epithelium with secretory function such as prostate. βV-tubulin was also specifically expressed in pancreatic islets and intratubular germ cell neoplasia, where it may have diagnostic utility. Initial studies in breast, lung and ovarian cancers indicated aberrant expression of βV-tubulin, suggesting that this isoform may be associated with tumorigenesis. Thus, βV-tubulin expression is a potentially promising prognostic marker of malignancy.

Original languageEnglish (US)
Pages (from-to)566-576
Number of pages11
JournalCytoskeleton
Volume69
Issue number8
DOIs
StatePublished - Aug 2012

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Tubulin
Antibodies
Muscle Cells
Immunohistochemistry
Neoplasms
Pancreatic Ducts
Bile Ducts
Islets of Langerhans
Immunoblotting
Drug Resistance
Germ Cells
Ovarian Neoplasms
Fluorescent Antibody Technique
Prostate
Mammals
Lung Neoplasms
Protein Isoforms
Carcinogenesis
Epithelium
Endothelial Cells

Keywords

  • βV-tubulin
  • Immunohistochemistry, cancer
  • Microtubules
  • Tubulin isotypes

ASJC Scopus subject areas

  • Cell Biology
  • Structural Biology

Cite this

Characterization of a human βv-tubulin antibody and expression of this isotype in normal and malignant human tissue. / Chao, Suzan K.; Wang, Yihong; Verdier-Pinard, Pascal; Yang, Chia-Ping H.; Liu, Lingling; Rodriguez-Gabin, Alicia; McDaid, Hayley M.; Band Horwitz, Susan.

In: Cytoskeleton, Vol. 69, No. 8, 08.2012, p. 566-576.

Research output: Contribution to journalArticle

Chao, Suzan K. ; Wang, Yihong ; Verdier-Pinard, Pascal ; Yang, Chia-Ping H. ; Liu, Lingling ; Rodriguez-Gabin, Alicia ; McDaid, Hayley M. ; Band Horwitz, Susan. / Characterization of a human βv-tubulin antibody and expression of this isotype in normal and malignant human tissue. In: Cytoskeleton. 2012 ; Vol. 69, No. 8. pp. 566-576.
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