Characterization and regulation of rat liver UDP glucuronosyltransferases

P. I. Mackenzie, Jayanta Roy-Chowdhury, J. R. Chowdhury

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

1. The use of DNA recombinant techniques to study UDP glucuronosyltransferases is reviewed. 2. The cloning and sequencing of the cDNAs to five forms of UDP glucuronosyltransferase demonstrated that these proteins are encoded by a superfamily of genes. 3. The substrate specificities of four isozymes have been analysed by expression of their corresponding cDNAs in cell culture. 4. Analysis of liver mRNA levels using radiolabelled DNA probes demonstrated that one isozyme, UDPGTr-2, is elevated by phenobarbital whereas a second isozyme, 4-nitrophenol GT is elevated by 3-methylcholanthrene. The levels of other isozymes are unaffected by these two prototypic inducers of drug metabolizing enzymes. 5. The level of 4-nitrophenol GT mRNA is elevated 10-15-fold in hyperplastic nodules in livers of rats treated with 2-acetylaminofluorene. Similar increases were not observed for mRNAs encoding other forms of UDP glucuronosyltransferase.

Original languageEnglish (US)
Pages (from-to)501-504
Number of pages4
JournalClinical and Experimental Pharmacology and Physiology
Volume16
Issue number6
StatePublished - 1989
Externally publishedYes

Fingerprint

Glucuronosyltransferase
Isoenzymes
Liver
Messenger RNA
Complementary DNA
2-Acetylaminofluorene
Methylcholanthrene
Recombinant DNA
DNA Probes
Phenobarbital
Substrate Specificity
Organism Cloning
Cell Culture Techniques
Enzymes
Pharmaceutical Preparations
Genes
Proteins
4-nitrophenol

ASJC Scopus subject areas

  • Physiology
  • Pharmacology (medical)
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Characterization and regulation of rat liver UDP glucuronosyltransferases. / Mackenzie, P. I.; Roy-Chowdhury, Jayanta; Chowdhury, J. R.

In: Clinical and Experimental Pharmacology and Physiology, Vol. 16, No. 6, 1989, p. 501-504.

Research output: Contribution to journalArticle

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