TY - JOUR
T1 - Characterization and genomic mapping of chimeric ERV9 endogenous retroviruses-host gene transcripts
AU - Strazzullo, Maria
AU - Parisi, Tiziana
AU - Di Cristofano, Antonio
AU - Mariano Rocchi, Rocchi
AU - La Mantia, Girolama
N1 - Funding Information:
We thank Miss R. Terracciano for skillful technical help. This work was paid for by grants from European Community (EC Grant no. Gene-CTP3-0019), the Italian Association for Cancer Research (AIRC) and from the AIDS program of the Istituto Superiore di Sanità (Rome) and MURST to G.L.M.; M.S. is a recipient of an AIRC fellowship.
PY - 1998/1/5
Y1 - 1998/1/5
N2 - ERV9 is a low repeated family of human endogenous retroviral elements, which has close to 50 members, in addition to at least 4000 solitary LTRs. Previous work has shown that randomly selected LTRs can promote transcription of reporter genes, raising the possibility that these sequences may affect the expression of adjacent cellular genes. We performed Northern blot experiments using sequences from ERV9-LTR, and we observed a different pattern of expression in several different hemopoietic tumor cell lines. It is possible that by the result of a somatic integration event, or by virtue of their original dispersal in the genome, ERV9-LTRs may specifically induce the expression of different cellular sequences in different cell lineages. Here, we describe the identification and analysis of four chimeric cDNA clones isolated from the T-lymphoma Peer cell line, having a structure consistent with transcription initiation from an ERV9-LTR. All the cDNA clones represent transcripts derived from unique cellular sequences. We also report the genomic localization of these cDNA clones.
AB - ERV9 is a low repeated family of human endogenous retroviral elements, which has close to 50 members, in addition to at least 4000 solitary LTRs. Previous work has shown that randomly selected LTRs can promote transcription of reporter genes, raising the possibility that these sequences may affect the expression of adjacent cellular genes. We performed Northern blot experiments using sequences from ERV9-LTR, and we observed a different pattern of expression in several different hemopoietic tumor cell lines. It is possible that by the result of a somatic integration event, or by virtue of their original dispersal in the genome, ERV9-LTRs may specifically induce the expression of different cellular sequences in different cell lineages. Here, we describe the identification and analysis of four chimeric cDNA clones isolated from the T-lymphoma Peer cell line, having a structure consistent with transcription initiation from an ERV9-LTR. All the cDNA clones represent transcripts derived from unique cellular sequences. We also report the genomic localization of these cDNA clones.
KW - Human-rodent cell hybrids
KW - LTR
KW - Repeated sequences
KW - Retrotransposition
UR - http://www.scopus.com/inward/record.url?scp=0032484602&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032484602&partnerID=8YFLogxK
U2 - 10.1016/S0378-1119(97)00568-4
DO - 10.1016/S0378-1119(97)00568-4
M3 - Article
C2 - 9461418
AN - SCOPUS:0032484602
SN - 0378-1119
VL - 206
SP - 77
EP - 83
JO - Gene
JF - Gene
IS - 1
ER -