Characterization and fine localization of two new genes in Xq28 using the genomic sequence/EST database screening approach

Sara Faranda, Annalisa Frattini, Ileana Zucchi, Cristina Patrosso, Luciano Milanesi, Cristina Montagna, Paolo Vezzoni

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

Two new genes were identified and mapped by searching the EST databases with genomic sequences obtained from putative CpG islands of the rodent- human hybrid X3000. Previous mapping of these CpG islands in the proximity of the host cell factor (HCFC1) and GdX genes automatically localized these two new genes to Xq28 in the interval between the L1 cell adhesion molecule (L1CAM) and the glucose-6-phosphate dehydrogenase (G6PD) loci. Both genes are relatively short, contain an ORF of 261 and 105 amino acids, respectively, and are ubiquitously expressed. Combining sequencing of selected CpG islands, derived from hybrids containing small portions of the human genome, with an EST database search is an easy method of identifying and mapping new genes to specific regions of the genome.

Original languageEnglish (US)
Pages (from-to)323-327
Number of pages5
JournalGenomics
Volume34
Issue number3
DOIs
Publication statusPublished - Jun 15 1996
Externally publishedYes

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ASJC Scopus subject areas

  • Genetics

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