Characterization and fine localization of two new genes in Xq28 using the genomic sequence/EST database screening approach

Sara Faranda, Annalisa Frattini, Ileana Zucchi, Cristina Patrosso, Luciano Milanesi, Cristina Montagna, Paolo Vezzoni

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Two new genes were identified and mapped by searching the EST databases with genomic sequences obtained from putative CpG islands of the rodent- human hybrid X3000. Previous mapping of these CpG islands in the proximity of the host cell factor (HCFC1) and GdX genes automatically localized these two new genes to Xq28 in the interval between the L1 cell adhesion molecule (L1CAM) and the glucose-6-phosphate dehydrogenase (G6PD) loci. Both genes are relatively short, contain an ORF of 261 and 105 amino acids, respectively, and are ubiquitously expressed. Combining sequencing of selected CpG islands, derived from hybrids containing small portions of the human genome, with an EST database search is an easy method of identifying and mapping new genes to specific regions of the genome.

Original languageEnglish (US)
Pages (from-to)323-327
Number of pages5
JournalGenomics
Volume34
Issue number3
DOIs
StatePublished - Jun 15 1996
Externally publishedYes

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Expressed Sequence Tags
CpG Islands
Databases
Genes
Neural Cell Adhesion Molecule L1
Glucosephosphate Dehydrogenase
Chromosome Mapping
Human Genome
Open Reading Frames
Rodentia
Genome
Amino Acids

ASJC Scopus subject areas

  • Genetics

Cite this

Characterization and fine localization of two new genes in Xq28 using the genomic sequence/EST database screening approach. / Faranda, Sara; Frattini, Annalisa; Zucchi, Ileana; Patrosso, Cristina; Milanesi, Luciano; Montagna, Cristina; Vezzoni, Paolo.

In: Genomics, Vol. 34, No. 3, 15.06.1996, p. 323-327.

Research output: Contribution to journalArticle

Faranda, Sara ; Frattini, Annalisa ; Zucchi, Ileana ; Patrosso, Cristina ; Milanesi, Luciano ; Montagna, Cristina ; Vezzoni, Paolo. / Characterization and fine localization of two new genes in Xq28 using the genomic sequence/EST database screening approach. In: Genomics. 1996 ; Vol. 34, No. 3. pp. 323-327.
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