Chapter 27 Cell Biology of mRNA Decay

David Grünwald, Robert H. Singer, Kevin Czaplinski

Research output: Chapter in Book/Report/Conference proceedingChapter

20 Scopus citations

Abstract

Studying single mRNA molecules has added new dimensions to our understanding of gene expression and the life cycle of mRNA in cells. Advances in microscopes and detection technology have opened access to single molecule research to most researchers interested in molecular biology. Here we provide an overview technique for single molecule studies of RNA in either fixed samples or in living cells. As part of a volume on mRNA turnover, it is increasingly relevant, because many of the recent advances in studies of mRNA turnover have suggested that there is non-homogeneous distribution of turnover factors in the cell. For this reason, understanding of spatial relationships between mRNA and mRNA turnover factors should enrich our understanding of this process.

Original languageEnglish (US)
Title of host publicationRNA Turnover in Eukaryotes
Subtitle of host publicationNucleases, Pathways and Anaylsis of mRNA Decay
EditorsLynne Maquat, Megerditch Kiledjian
Pages553-577
Number of pages25
DOIs
StatePublished - Dec 24 2008

Publication series

NameMethods in Enzymology
Volume448
ISSN (Print)0076-6879

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ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Grünwald, D., Singer, R. H., & Czaplinski, K. (2008). Chapter 27 Cell Biology of mRNA Decay. In L. Maquat, & M. Kiledjian (Eds.), RNA Turnover in Eukaryotes: Nucleases, Pathways and Anaylsis of mRNA Decay (pp. 553-577). (Methods in Enzymology; Vol. 448). https://doi.org/10.1016/S0076-6879(08)02627-X