Chapter 27 Cell Biology of mRNA Decay

David Grünwald, Robert H. Singer, Kevin Czaplinski

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Studying single mRNA molecules has added new dimensions to our understanding of gene expression and the life cycle of mRNA in cells. Advances in microscopes and detection technology have opened access to single molecule research to most researchers interested in molecular biology. Here we provide an overview technique for single molecule studies of RNA in either fixed samples or in living cells. As part of a volume on mRNA turnover, it is increasingly relevant, because many of the recent advances in studies of mRNA turnover have suggested that there is non-homogeneous distribution of turnover factors in the cell. For this reason, understanding of spatial relationships between mRNA and mRNA turnover factors should enrich our understanding of this process.

Original languageEnglish (US)
Pages (from-to)553-577
Number of pages25
JournalMethods in Enzymology
Volume448
DOIs
StatePublished - 2008

Fingerprint

Cytology
RNA Stability
Cell Biology
Messenger RNA
Molecules
Molecular biology
Life Cycle Stages
Gene expression
Life cycle
Molecular Biology
Microscopes
Cells
Research Personnel
RNA
Technology
Gene Expression
Research

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Chapter 27 Cell Biology of mRNA Decay. / Grünwald, David; Singer, Robert H.; Czaplinski, Kevin.

In: Methods in Enzymology, Vol. 448, 2008, p. 553-577.

Research output: Contribution to journalArticle

Grünwald, David ; Singer, Robert H. ; Czaplinski, Kevin. / Chapter 27 Cell Biology of mRNA Decay. In: Methods in Enzymology. 2008 ; Vol. 448. pp. 553-577.
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