Chapter 19 Methods to Monitor Chaperone-Mediated Autophagy

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Abstract

Chaperone-mediated autophagy (CMA) is a selective type of autophagy responsible for the lysosomal degradation of soluble cytosolic proteins. In contrast to other forms of autophagy where cargo is sequestered and delivered to lysosomes through membrane fusion/excision, CMA substrates reach the lysosomal lumen after direct translocation across the lysosomal membrane. CMA is part of the cellular quality control systems and as such, essential for the cellular response to stress. CMA activity decreases with age, likely contributing to the accumulation of altered proteins characteristic in tissues from old organisms. Furthermore, impairment of CMA underlies the pathogenesis of certain human pathologies such as neurodegenerative disorders. These findings have drawn renewed attention to CMA and a growing interest in the measurement of changes in CMA activity under different physiological and pathological conditions. In this chapter we review the different experimental approaches used to assess CMA activity both in cells in culture and in different organs from animals.

Original languageEnglish (US)
Pages (from-to)297-324
Number of pages28
JournalMethods in Enzymology
Volume451
Issue numberC
DOIs
StatePublished - 2009

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Autophagy
Membranes
Pathology
Quality control
Animals
Proteins
Fusion reactions
Tissue
Control systems
Degradation
Substrates
Animal Structures
Membrane Fusion
Lysosomes
Neurodegenerative Diseases
Quality Control
Cell Culture Techniques

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Chapter 19 Methods to Monitor Chaperone-Mediated Autophagy. / Kaushik, Susmita; Cuervo, Ana Maria.

In: Methods in Enzymology, Vol. 451, No. C, 2009, p. 297-324.

Research output: Contribution to journalArticle

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