Channels formed by botulinum, tetanus, and diphtheria toxins in planar lipid bilayers: Relevance to translocation of proteins across membranes

D. H. Hoch, M. Romero-Mira, B. E. Ehrlich, A. Finkelstein, B. R. DasGupta, L. L. Simpson

Research output: Contribution to journalArticle

236 Citations (Scopus)

Abstract

The heavy chains of both botulinum neurotoxin type B and tetanus toxin form channels in planar bilayer membranes. These channels have pH-dependent and voltage-dependent properties that are remarkably similar to those previously described for diphtheria toxin. Selectivity experiments with anions and cations show that the channels formed by the heavy chains of all three toxins are large; thus, these channels could serve as 'tunnel proteins' for translocation of active peptide fragments. These findings support the hypothesis that the active fragments of botulinum neurotoxin and tetanus toxin, like that of diphtheria toxin, are translocated across the membranes of acidic vesicles.

Original languageEnglish (US)
Pages (from-to)1692-1696
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume82
Issue number6
DOIs
StatePublished - 1985
Externally publishedYes

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Tetanus Toxin
Diphtheria Toxin
Botulinum Toxins
Lipid Bilayers
Protein Transport
Membrane Proteins
Peptide Fragments
Membranes
Neurotoxins
Anions
Cations
rimabotulinumtoxinB

ASJC Scopus subject areas

  • Genetics
  • General

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Channels formed by botulinum, tetanus, and diphtheria toxins in planar lipid bilayers : Relevance to translocation of proteins across membranes. / Hoch, D. H.; Romero-Mira, M.; Ehrlich, B. E.; Finkelstein, A.; DasGupta, B. R.; Simpson, L. L.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 82, No. 6, 1985, p. 1692-1696.

Research output: Contribution to journalArticle

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