Changes with aging in human endothelial cell cytoskeleton

C. S P Jenkins, P. B. Gordon, Victor Bernard Hatcher, E. G. Puszkin

Research output: Contribution to journalArticle

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Abstract

Human umbilical vein endothelial cells were cultured in the presence or absence of acidic fibroblast growth factor. As these cells reach senescence, changes occur in the morphology, organization and cellular distribution of the cytoskeletal components. This was most marked with α-actinin and vinculin. In early-passaged cells α-actinin was associated with the stress fibers with a periodic appearance. In later-passaged cells α-actinin was found to be more diffuse in its distribution and with an apparent loss of periodicity. The organization of vinculin in early passaged cells was found to be concentrated at the ends of the stress fibers, whereas in later-passaged cells, it was associated at discrete regions of the membrane. The distribution of the cytoskeletal components in early-passaged cells, cultured in part in the absence of an acidic fibroblast growth factor, resemble later-passaged cells cultured in the presence of growth factor. The changes observed in the organization of vinculin in senescent cells and in early-passaged cells cultured in the absence of acidic fibroblast growth factors demonstrated than the acidic fibroblast growth factor promotes cell adhesion and prevents, to a greater extent, cell detachment.

Original languageEnglish (US)
Pages (from-to)313-320
Number of pages8
JournalJournal of Histotechnology
Volume12
Issue number4
StatePublished - 1989

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Cytoskeleton
Fibroblast Growth Factor 1
Endothelial Cells
Vinculin
Actinin
Cultured Cells
Stress Fibers
Cell Aging
Human Umbilical Vein Endothelial Cells
Periodicity
Cell Adhesion
Intercellular Signaling Peptides and Proteins
Membranes

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Jenkins, C. S. P., Gordon, P. B., Hatcher, V. B., & Puszkin, E. G. (1989). Changes with aging in human endothelial cell cytoskeleton. Journal of Histotechnology, 12(4), 313-320.

Changes with aging in human endothelial cell cytoskeleton. / Jenkins, C. S P; Gordon, P. B.; Hatcher, Victor Bernard; Puszkin, E. G.

In: Journal of Histotechnology, Vol. 12, No. 4, 1989, p. 313-320.

Research output: Contribution to journalArticle

Jenkins, CSP, Gordon, PB, Hatcher, VB & Puszkin, EG 1989, 'Changes with aging in human endothelial cell cytoskeleton', Journal of Histotechnology, vol. 12, no. 4, pp. 313-320.
Jenkins, C. S P ; Gordon, P. B. ; Hatcher, Victor Bernard ; Puszkin, E. G. / Changes with aging in human endothelial cell cytoskeleton. In: Journal of Histotechnology. 1989 ; Vol. 12, No. 4. pp. 313-320.
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