TY - JOUR
T1 - Changes in the G0 state of WI-38 fibroblasts at different times after confluence
AU - Augenlicht, L. H.
AU - Baserga, R.
N1 - Funding Information:
This work was supported by USPHS researchg rants CA-08373a nd CA-12923,a nd TrainingGrant(L.H.A.) CA-5222 from the NCI.
PY - 1974/12
Y1 - 1974/12
N2 - Some events in the prereplicative phase of WI-38 human diploid fibroblasts stimulated to proliferate are found to be a function of the length of time the cells have been quiescent. At 5 days after plating, when the cells first become confluent, the prereplicative phase upon stimulation by a nutritional change is relatively short, DNA synthesis begins at 8 h after stimulation, and there is no increase in chromatin template activity. At 9 days after plating the prereplicative phase of stimulated cells is lengthened to 14 h and there is an increase in chromatin template activity within 1 h of stimulation. Finally, in 18-day cells, the prereplicative phase is lengthened even further to 20 h, and there is a lag after stimulation before the increase in chromatin template activity. It is proposed that confluent WI-38 cells initially arrest in G 1, subsequently pass into G 0, and continue to go deeper into G 0 as they remain quiescent.
AB - Some events in the prereplicative phase of WI-38 human diploid fibroblasts stimulated to proliferate are found to be a function of the length of time the cells have been quiescent. At 5 days after plating, when the cells first become confluent, the prereplicative phase upon stimulation by a nutritional change is relatively short, DNA synthesis begins at 8 h after stimulation, and there is no increase in chromatin template activity. At 9 days after plating the prereplicative phase of stimulated cells is lengthened to 14 h and there is an increase in chromatin template activity within 1 h of stimulation. Finally, in 18-day cells, the prereplicative phase is lengthened even further to 20 h, and there is a lag after stimulation before the increase in chromatin template activity. It is proposed that confluent WI-38 cells initially arrest in G 1, subsequently pass into G 0, and continue to go deeper into G 0 as they remain quiescent.
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U2 - 10.1016/0014-4827(74)90789-7
DO - 10.1016/0014-4827(74)90789-7
M3 - Article
C2 - 4616835
AN - SCOPUS:0016144499
SN - 0014-4827
VL - 89
SP - 255
EP - 262
JO - Experimental Cell Research
JF - Experimental Cell Research
IS - 2
ER -