Changes in muscle mRNAs for hexokinase, phosphofructokinase-1 and glycogen synthase in acute and persistent hypoglycemia induced by tolbutamide in chickens

Yoshinori Seki, Kan Sato, Yukio Akiba

Research output: Contribution to journalArticle

10 Scopus citations


To elucidate the specificity of glucose metabolism in chicken skeletal muscle, changes in mRNA levels of hexokinase I (HKI), hexokinase II (HKII), phosphofructokinase-1 (PFK-1) and glycogen synthase (GS) were characterized in acute and persistent hypoglycemia induced by tolbutamide administration. In acute hypoglycemia, induced by a single dose of tolbutamide (100 mg/kg body mass), HKII, PFK-1 and GS mRNA levels remained unchanged; however, levels of HKI mRNA and glucose transporter 1 (GLUT1) were significantly increased 4 h after administration. In persistent hypoglycemia, induced by sequential administration of tolbutamide (100 mg/kg body mass) 3 times a day for 5 days, GS mRNA was significantly increased at day 5, while HKI, HKII and PFK-1 mRNA levels remained unchanged. These results suggest that HKI is responsible for glucose transport into skeletal muscle in acute hypoglycemia and that glucose preferentially enters the glycogenic pathway before the glycolytic pathway in persistently hypoglycemic chickens.

Original languageEnglish (US)
Pages (from-to)201-208
Number of pages8
JournalComparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
Issue number2
StatePublished - Oct 2005



  • Chicken
  • Glycogen synthase (GS)
  • Hexokinase (HK)
  • Hypoglycemia
  • Phosphofructokinase-1 (PFK-1)
  • Tolbutamide
  • mRNA

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Molecular Biology

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