TY - JOUR
T1 - Changes in laminin during recovery from postischemic acute renal failure in rats
AU - Lin, Jen Jar
AU - Partin, Jacqueline
AU - Kaskel, Frederick J.
N1 - Copyright:
Copyright 2007 Elsevier B.V., All rights reserved.
PY - 1996/9
Y1 - 1996/9
N2 - Changes in the immunoreactivity of laminin have been demonstrated in a variety of tissues undergoing injury-regeneration process and during nephrogenesis. Studies in vitro revealed that laminin is important in many cellular functions such as growth, differentiation, and cell communication. This study examined the changes of laminin in the extracellular matrix (ECM) of proximal straight tubules (PST) in the outer stripe of the outer medulla after renal ischemia, an area where most morphological damage occurred. Anesthetized male Sprague-Dawley adult rats underwent a 30-min temporary occlusion of the left renal artery. The inulin clearance (C(in)) of the postischemic left kidneys was significantly decreased at 30 min, 1 day, 2 days, and 5 days after the injury, and at 7 and 10 days after the injury it rose to a level not significantly different from the sham-operated controls. Using immunogold electron microscopy, the density of immunoreactive laminin (gold-conjugated laminin grains/μm2 ECM area) in PST where flattened PST cells were present was decreased at 1 day and 2 days, and at 5 days, a time when the renal function was still depressed, it returned to a level not significantly different from that in controls. These changes were not observed in those PST or proximal convoluted tubules in which morphology was intact. Further studies are needed to determine if these changes in laminin of damaged PST cells have any significant role during their recovery from the ischemic injury.
AB - Changes in the immunoreactivity of laminin have been demonstrated in a variety of tissues undergoing injury-regeneration process and during nephrogenesis. Studies in vitro revealed that laminin is important in many cellular functions such as growth, differentiation, and cell communication. This study examined the changes of laminin in the extracellular matrix (ECM) of proximal straight tubules (PST) in the outer stripe of the outer medulla after renal ischemia, an area where most morphological damage occurred. Anesthetized male Sprague-Dawley adult rats underwent a 30-min temporary occlusion of the left renal artery. The inulin clearance (C(in)) of the postischemic left kidneys was significantly decreased at 30 min, 1 day, 2 days, and 5 days after the injury, and at 7 and 10 days after the injury it rose to a level not significantly different from the sham-operated controls. Using immunogold electron microscopy, the density of immunoreactive laminin (gold-conjugated laminin grains/μm2 ECM area) in PST where flattened PST cells were present was decreased at 1 day and 2 days, and at 5 days, a time when the renal function was still depressed, it returned to a level not significantly different from that in controls. These changes were not observed in those PST or proximal convoluted tubules in which morphology was intact. Further studies are needed to determine if these changes in laminin of damaged PST cells have any significant role during their recovery from the ischemic injury.
KW - Acute renal failure
KW - Extracellular matrix
KW - Ischemia
KW - Laminin
KW - Proximal straight tubule
KW - Regeneration
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M3 - Article
C2 - 8931983
AN - SCOPUS:0029802721
SN - 1018-7782
VL - 4
SP - 279
EP - 285
JO - Experimental Nephrology
JF - Experimental Nephrology
IS - 5
ER -