Changes in GIP gene expression following bariatric surgery

Erin Moran-Atkin, Fred Brody, Sidney W. Fu, Marcos Rojkind

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Background: After bariatric surgery, there is a significant improvement in type 2 diabetes (T2D). T2D has been linked to incretins, including glucose-dependent insulinotropic polypeptide (GIP). Analysis of bariatric surgery patients may help to understand the link between GIP and T2D. Methods: Twenty-three morbidly obese patients underwent Roux-en-Y gastric bypass (RYGB) or gastric banding. Overall, there were 12 RYGB (5 T2D; 7 nondiabetic) patients and 11 gastric band (7 T2D; 4 nondiabetic) patients. Preoperative and postoperative blood samples were collected. Total RNA was extracted, cDNA synthesized, and real-time quantitative PCR were used to quantify gene expression. Student's t test was used for statistical analysis. Results: Postoperatively, T2D resolved or improved in 83.3 % (10/12) of the diabetic patients. Six (4 RYGB, 2 bands) patients discontinued hypoglycemic medications and four (3 RYGB, 1 band) patients discontinued the majority of their hypoglycemic agents. The remaining two diabetic patients (bands) showed no improvement. Postoperative GIP gene expression increased 4.36-fold (p = 0.02) in diabetic RYGB patients, whereas diabetic band patients increased 1.4-fold (p = 0.25). All diabetic patients with either resolution or improvement of T2D, had a 3.4-fold increase (p = 0.01) but nonresponders decreased 0.69-fold (p = 0.41). Nondiabetic RYGB patients increased 2.21-fold (p = 0.07) versus a 0.81-fold (p = 0.37) decrease of nondiabetic band patients. Conclusions: This is one of the initial studies that show a significant increase in GIP gene expression following a RYGB. This increase correlates with the clinical resolution of T2D. The anatomical changes after RYGB may account for these changes. Based on this data, GIP may be a key peptide in the "foregut hypothesis" for resolution of T2D.

Original languageEnglish (US)
Pages (from-to)2492-2497
Number of pages6
JournalSurgical Endoscopy and Other Interventional Techniques
Volume27
Issue number7
DOIs
StatePublished - Jul 2013
Externally publishedYes

Fingerprint

Bariatric Surgery
Gastric Bypass
Gene Expression
Glucose
Type 2 Diabetes Mellitus
Peptides
Hypoglycemic Agents
Stomach
Incretins
Real-Time Polymerase Chain Reaction
Complementary DNA

Keywords

  • Bariatric
  • Obesity

ASJC Scopus subject areas

  • Surgery

Cite this

Changes in GIP gene expression following bariatric surgery. / Moran-Atkin, Erin; Brody, Fred; Fu, Sidney W.; Rojkind, Marcos.

In: Surgical Endoscopy and Other Interventional Techniques, Vol. 27, No. 7, 07.2013, p. 2492-2497.

Research output: Contribution to journalArticle

Moran-Atkin, Erin ; Brody, Fred ; Fu, Sidney W. ; Rojkind, Marcos. / Changes in GIP gene expression following bariatric surgery. In: Surgical Endoscopy and Other Interventional Techniques. 2013 ; Vol. 27, No. 7. pp. 2492-2497.
@article{103d7a4d178c418a91e1a5b613861b6d,
title = "Changes in GIP gene expression following bariatric surgery",
abstract = "Background: After bariatric surgery, there is a significant improvement in type 2 diabetes (T2D). T2D has been linked to incretins, including glucose-dependent insulinotropic polypeptide (GIP). Analysis of bariatric surgery patients may help to understand the link between GIP and T2D. Methods: Twenty-three morbidly obese patients underwent Roux-en-Y gastric bypass (RYGB) or gastric banding. Overall, there were 12 RYGB (5 T2D; 7 nondiabetic) patients and 11 gastric band (7 T2D; 4 nondiabetic) patients. Preoperative and postoperative blood samples were collected. Total RNA was extracted, cDNA synthesized, and real-time quantitative PCR were used to quantify gene expression. Student's t test was used for statistical analysis. Results: Postoperatively, T2D resolved or improved in 83.3 {\%} (10/12) of the diabetic patients. Six (4 RYGB, 2 bands) patients discontinued hypoglycemic medications and four (3 RYGB, 1 band) patients discontinued the majority of their hypoglycemic agents. The remaining two diabetic patients (bands) showed no improvement. Postoperative GIP gene expression increased 4.36-fold (p = 0.02) in diabetic RYGB patients, whereas diabetic band patients increased 1.4-fold (p = 0.25). All diabetic patients with either resolution or improvement of T2D, had a 3.4-fold increase (p = 0.01) but nonresponders decreased 0.69-fold (p = 0.41). Nondiabetic RYGB patients increased 2.21-fold (p = 0.07) versus a 0.81-fold (p = 0.37) decrease of nondiabetic band patients. Conclusions: This is one of the initial studies that show a significant increase in GIP gene expression following a RYGB. This increase correlates with the clinical resolution of T2D. The anatomical changes after RYGB may account for these changes. Based on this data, GIP may be a key peptide in the {"}foregut hypothesis{"} for resolution of T2D.",
keywords = "Bariatric, Obesity",
author = "Erin Moran-Atkin and Fred Brody and Fu, {Sidney W.} and Marcos Rojkind",
year = "2013",
month = "7",
doi = "10.1007/s00464-012-2764-8",
language = "English (US)",
volume = "27",
pages = "2492--2497",
journal = "Surgical Endoscopy and Other Interventional Techniques",
issn = "0930-2794",
publisher = "Springer New York",
number = "7",

}

TY - JOUR

T1 - Changes in GIP gene expression following bariatric surgery

AU - Moran-Atkin, Erin

AU - Brody, Fred

AU - Fu, Sidney W.

AU - Rojkind, Marcos

PY - 2013/7

Y1 - 2013/7

N2 - Background: After bariatric surgery, there is a significant improvement in type 2 diabetes (T2D). T2D has been linked to incretins, including glucose-dependent insulinotropic polypeptide (GIP). Analysis of bariatric surgery patients may help to understand the link between GIP and T2D. Methods: Twenty-three morbidly obese patients underwent Roux-en-Y gastric bypass (RYGB) or gastric banding. Overall, there were 12 RYGB (5 T2D; 7 nondiabetic) patients and 11 gastric band (7 T2D; 4 nondiabetic) patients. Preoperative and postoperative blood samples were collected. Total RNA was extracted, cDNA synthesized, and real-time quantitative PCR were used to quantify gene expression. Student's t test was used for statistical analysis. Results: Postoperatively, T2D resolved or improved in 83.3 % (10/12) of the diabetic patients. Six (4 RYGB, 2 bands) patients discontinued hypoglycemic medications and four (3 RYGB, 1 band) patients discontinued the majority of their hypoglycemic agents. The remaining two diabetic patients (bands) showed no improvement. Postoperative GIP gene expression increased 4.36-fold (p = 0.02) in diabetic RYGB patients, whereas diabetic band patients increased 1.4-fold (p = 0.25). All diabetic patients with either resolution or improvement of T2D, had a 3.4-fold increase (p = 0.01) but nonresponders decreased 0.69-fold (p = 0.41). Nondiabetic RYGB patients increased 2.21-fold (p = 0.07) versus a 0.81-fold (p = 0.37) decrease of nondiabetic band patients. Conclusions: This is one of the initial studies that show a significant increase in GIP gene expression following a RYGB. This increase correlates with the clinical resolution of T2D. The anatomical changes after RYGB may account for these changes. Based on this data, GIP may be a key peptide in the "foregut hypothesis" for resolution of T2D.

AB - Background: After bariatric surgery, there is a significant improvement in type 2 diabetes (T2D). T2D has been linked to incretins, including glucose-dependent insulinotropic polypeptide (GIP). Analysis of bariatric surgery patients may help to understand the link between GIP and T2D. Methods: Twenty-three morbidly obese patients underwent Roux-en-Y gastric bypass (RYGB) or gastric banding. Overall, there were 12 RYGB (5 T2D; 7 nondiabetic) patients and 11 gastric band (7 T2D; 4 nondiabetic) patients. Preoperative and postoperative blood samples were collected. Total RNA was extracted, cDNA synthesized, and real-time quantitative PCR were used to quantify gene expression. Student's t test was used for statistical analysis. Results: Postoperatively, T2D resolved or improved in 83.3 % (10/12) of the diabetic patients. Six (4 RYGB, 2 bands) patients discontinued hypoglycemic medications and four (3 RYGB, 1 band) patients discontinued the majority of their hypoglycemic agents. The remaining two diabetic patients (bands) showed no improvement. Postoperative GIP gene expression increased 4.36-fold (p = 0.02) in diabetic RYGB patients, whereas diabetic band patients increased 1.4-fold (p = 0.25). All diabetic patients with either resolution or improvement of T2D, had a 3.4-fold increase (p = 0.01) but nonresponders decreased 0.69-fold (p = 0.41). Nondiabetic RYGB patients increased 2.21-fold (p = 0.07) versus a 0.81-fold (p = 0.37) decrease of nondiabetic band patients. Conclusions: This is one of the initial studies that show a significant increase in GIP gene expression following a RYGB. This increase correlates with the clinical resolution of T2D. The anatomical changes after RYGB may account for these changes. Based on this data, GIP may be a key peptide in the "foregut hypothesis" for resolution of T2D.

KW - Bariatric

KW - Obesity

UR - http://www.scopus.com/inward/record.url?scp=84884909477&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84884909477&partnerID=8YFLogxK

U2 - 10.1007/s00464-012-2764-8

DO - 10.1007/s00464-012-2764-8

M3 - Article

C2 - 23306619

AN - SCOPUS:84884909477

VL - 27

SP - 2492

EP - 2497

JO - Surgical Endoscopy and Other Interventional Techniques

JF - Surgical Endoscopy and Other Interventional Techniques

SN - 0930-2794

IS - 7

ER -