Changes in axonally transported proteins in the rat visual system following systemic methyl mercury exposure

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

In an effort to understand the effect of methylmercury on protein synthesis and axonal transport, we have analysed the composition and rate of axonally transported proteins in the retinal ganglion cells of the mature rat. By means of scintillation spectrometry and autoradiography, it was established that systemic exposure to 4 mgHg/kg/day for four to six days, or twelve days, resulted in an increased rate and volume of transported protein-bound radioactivity in the visual system of the mature rat. In an effort to characterize these changes, the composition of transported polypeptides was analysed by means of SDS polyacrylamide gel electrophoresis. Selective changes in the composition of transported polypeptides were evident. These changes of a small subset of proteins known as GAPs (growth-associated proteins) are consistent with the suggestion that they may have been involved in growth-specific functions during the early stages of methyl mercury exposure. We conclude that, during this period, retinal ganglion cells may express growth-related genes and engage in regenerative processes.

Original languageEnglish (US)
Pages (from-to)151-157
Number of pages7
JournalActa Pharmacologica et Toxicologica
Volume59
Issue number2
StatePublished - 1986
Externally publishedYes

Fingerprint

Mercury
Rats
Retinal Ganglion Cells
Proteins
Growth
Peptides
Axonal Transport
Chemical analysis
Protein Transport
Autoradiography
Radioactivity
Scintillation
Polyacrylamide Gel Electrophoresis
Spectrum Analysis
Electrophoresis
Spectrometry
Genes
Cells

ASJC Scopus subject areas

  • Pharmacology
  • Toxicology

Cite this

@article{36ff88a199e349bc86c3751cd2efe08f,
title = "Changes in axonally transported proteins in the rat visual system following systemic methyl mercury exposure",
abstract = "In an effort to understand the effect of methylmercury on protein synthesis and axonal transport, we have analysed the composition and rate of axonally transported proteins in the retinal ganglion cells of the mature rat. By means of scintillation spectrometry and autoradiography, it was established that systemic exposure to 4 mgHg/kg/day for four to six days, or twelve days, resulted in an increased rate and volume of transported protein-bound radioactivity in the visual system of the mature rat. In an effort to characterize these changes, the composition of transported polypeptides was analysed by means of SDS polyacrylamide gel electrophoresis. Selective changes in the composition of transported polypeptides were evident. These changes of a small subset of proteins known as GAPs (growth-associated proteins) are consistent with the suggestion that they may have been involved in growth-specific functions during the early stages of methyl mercury exposure. We conclude that, during this period, retinal ganglion cells may express growth-related genes and engage in regenerative processes.",
author = "Michael Aschner",
year = "1986",
language = "English (US)",
volume = "59",
pages = "151--157",
journal = "Basic and Clinical Pharmacology and Toxicology",
issn = "1742-7835",
publisher = "Wiley-Blackwell",
number = "2",

}

TY - JOUR

T1 - Changes in axonally transported proteins in the rat visual system following systemic methyl mercury exposure

AU - Aschner, Michael

PY - 1986

Y1 - 1986

N2 - In an effort to understand the effect of methylmercury on protein synthesis and axonal transport, we have analysed the composition and rate of axonally transported proteins in the retinal ganglion cells of the mature rat. By means of scintillation spectrometry and autoradiography, it was established that systemic exposure to 4 mgHg/kg/day for four to six days, or twelve days, resulted in an increased rate and volume of transported protein-bound radioactivity in the visual system of the mature rat. In an effort to characterize these changes, the composition of transported polypeptides was analysed by means of SDS polyacrylamide gel electrophoresis. Selective changes in the composition of transported polypeptides were evident. These changes of a small subset of proteins known as GAPs (growth-associated proteins) are consistent with the suggestion that they may have been involved in growth-specific functions during the early stages of methyl mercury exposure. We conclude that, during this period, retinal ganglion cells may express growth-related genes and engage in regenerative processes.

AB - In an effort to understand the effect of methylmercury on protein synthesis and axonal transport, we have analysed the composition and rate of axonally transported proteins in the retinal ganglion cells of the mature rat. By means of scintillation spectrometry and autoradiography, it was established that systemic exposure to 4 mgHg/kg/day for four to six days, or twelve days, resulted in an increased rate and volume of transported protein-bound radioactivity in the visual system of the mature rat. In an effort to characterize these changes, the composition of transported polypeptides was analysed by means of SDS polyacrylamide gel electrophoresis. Selective changes in the composition of transported polypeptides were evident. These changes of a small subset of proteins known as GAPs (growth-associated proteins) are consistent with the suggestion that they may have been involved in growth-specific functions during the early stages of methyl mercury exposure. We conclude that, during this period, retinal ganglion cells may express growth-related genes and engage in regenerative processes.

UR - http://www.scopus.com/inward/record.url?scp=0022534493&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022534493&partnerID=8YFLogxK

M3 - Article

VL - 59

SP - 151

EP - 157

JO - Basic and Clinical Pharmacology and Toxicology

JF - Basic and Clinical Pharmacology and Toxicology

SN - 1742-7835

IS - 2

ER -