Cervicovaginal secretions contribute to innate resistance to herpes simplex virus infection

Minnie John, Marla J. Keller, Ehsan H. Fam, Natalia V. Cheshenko, Kathleen Hogarty, Andrea Kosowitz, Sylvan Wallenstein, Maria J. Carlucci, Ana C. Tuyama, Wuyyan Lu, Mary E. Klotman, Robert I. Lehrer, Betsy Herold

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

Defining and preserving the innate antiviral activity found in cervicovaginal secretions is critical. Cervicovaginal lavage (CVL) samples were obtained from 20 healthy women and evaluated for anti-herpes simplex virus (HSV) activity. CVL samples reduced HSV-2 yields by 23-fold (median), and the anti-HSV activity of CVL samples correlated with the concentration of human neutrophil peptides (HNP)-1-3. Both CVL samples and HNP-1-3 interacted with virus and prevented entry after binding. Substantially less protective activity was observed in CVL samples obtained from 20 human immunodeficiency virus-infected subjects, but the addition of CVL samples from healthy subjects enhanced the antiviral activity. The significance of the innate activity was further demonstrated by showing that CVL samples prevented murine genital herpes. Fourteen of 15 mice were protected from genital herpes if they were challenged with HSV-2 pretreated with CVL samples from healthy subjects. In contrast, all 15 mice challenged with untreated HSV-2 died. These findings are evidence that cervicovaginal secretions contribute to innate resistance to HSV-2 and identify defensins as contributors to this activity.

Original languageEnglish (US)
Pages (from-to)1731-1740
Number of pages10
JournalJournal of Infectious Diseases
Volume192
Issue number10
DOIs
StatePublished - Nov 15 2005
Externally publishedYes

Fingerprint

Therapeutic Irrigation
Virus Diseases
Simplexvirus
Human Herpesvirus 2
Herpes Genitalis
Antiviral Agents
Healthy Volunteers
Defensins
Virus Internalization
HIV

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Immunology

Cite this

Cervicovaginal secretions contribute to innate resistance to herpes simplex virus infection. / John, Minnie; Keller, Marla J.; Fam, Ehsan H.; Cheshenko, Natalia V.; Hogarty, Kathleen; Kosowitz, Andrea; Wallenstein, Sylvan; Carlucci, Maria J.; Tuyama, Ana C.; Lu, Wuyyan; Klotman, Mary E.; Lehrer, Robert I.; Herold, Betsy.

In: Journal of Infectious Diseases, Vol. 192, No. 10, 15.11.2005, p. 1731-1740.

Research output: Contribution to journalArticle

John, M, Keller, MJ, Fam, EH, Cheshenko, NV, Hogarty, K, Kosowitz, A, Wallenstein, S, Carlucci, MJ, Tuyama, AC, Lu, W, Klotman, ME, Lehrer, RI & Herold, B 2005, 'Cervicovaginal secretions contribute to innate resistance to herpes simplex virus infection', Journal of Infectious Diseases, vol. 192, no. 10, pp. 1731-1740. https://doi.org/10.1086/497168
John, Minnie ; Keller, Marla J. ; Fam, Ehsan H. ; Cheshenko, Natalia V. ; Hogarty, Kathleen ; Kosowitz, Andrea ; Wallenstein, Sylvan ; Carlucci, Maria J. ; Tuyama, Ana C. ; Lu, Wuyyan ; Klotman, Mary E. ; Lehrer, Robert I. ; Herold, Betsy. / Cervicovaginal secretions contribute to innate resistance to herpes simplex virus infection. In: Journal of Infectious Diseases. 2005 ; Vol. 192, No. 10. pp. 1731-1740.
@article{9bf3fbbb005e4fd3a8309ecb353d48ce,
title = "Cervicovaginal secretions contribute to innate resistance to herpes simplex virus infection",
abstract = "Defining and preserving the innate antiviral activity found in cervicovaginal secretions is critical. Cervicovaginal lavage (CVL) samples were obtained from 20 healthy women and evaluated for anti-herpes simplex virus (HSV) activity. CVL samples reduced HSV-2 yields by 23-fold (median), and the anti-HSV activity of CVL samples correlated with the concentration of human neutrophil peptides (HNP)-1-3. Both CVL samples and HNP-1-3 interacted with virus and prevented entry after binding. Substantially less protective activity was observed in CVL samples obtained from 20 human immunodeficiency virus-infected subjects, but the addition of CVL samples from healthy subjects enhanced the antiviral activity. The significance of the innate activity was further demonstrated by showing that CVL samples prevented murine genital herpes. Fourteen of 15 mice were protected from genital herpes if they were challenged with HSV-2 pretreated with CVL samples from healthy subjects. In contrast, all 15 mice challenged with untreated HSV-2 died. These findings are evidence that cervicovaginal secretions contribute to innate resistance to HSV-2 and identify defensins as contributors to this activity.",
author = "Minnie John and Keller, {Marla J.} and Fam, {Ehsan H.} and Cheshenko, {Natalia V.} and Kathleen Hogarty and Andrea Kosowitz and Sylvan Wallenstein and Carlucci, {Maria J.} and Tuyama, {Ana C.} and Wuyyan Lu and Klotman, {Mary E.} and Lehrer, {Robert I.} and Betsy Herold",
year = "2005",
month = "11",
day = "15",
doi = "10.1086/497168",
language = "English (US)",
volume = "192",
pages = "1731--1740",
journal = "Journal of Infectious Diseases",
issn = "0022-1899",
publisher = "Oxford University Press",
number = "10",

}

TY - JOUR

T1 - Cervicovaginal secretions contribute to innate resistance to herpes simplex virus infection

AU - John, Minnie

AU - Keller, Marla J.

AU - Fam, Ehsan H.

AU - Cheshenko, Natalia V.

AU - Hogarty, Kathleen

AU - Kosowitz, Andrea

AU - Wallenstein, Sylvan

AU - Carlucci, Maria J.

AU - Tuyama, Ana C.

AU - Lu, Wuyyan

AU - Klotman, Mary E.

AU - Lehrer, Robert I.

AU - Herold, Betsy

PY - 2005/11/15

Y1 - 2005/11/15

N2 - Defining and preserving the innate antiviral activity found in cervicovaginal secretions is critical. Cervicovaginal lavage (CVL) samples were obtained from 20 healthy women and evaluated for anti-herpes simplex virus (HSV) activity. CVL samples reduced HSV-2 yields by 23-fold (median), and the anti-HSV activity of CVL samples correlated with the concentration of human neutrophil peptides (HNP)-1-3. Both CVL samples and HNP-1-3 interacted with virus and prevented entry after binding. Substantially less protective activity was observed in CVL samples obtained from 20 human immunodeficiency virus-infected subjects, but the addition of CVL samples from healthy subjects enhanced the antiviral activity. The significance of the innate activity was further demonstrated by showing that CVL samples prevented murine genital herpes. Fourteen of 15 mice were protected from genital herpes if they were challenged with HSV-2 pretreated with CVL samples from healthy subjects. In contrast, all 15 mice challenged with untreated HSV-2 died. These findings are evidence that cervicovaginal secretions contribute to innate resistance to HSV-2 and identify defensins as contributors to this activity.

AB - Defining and preserving the innate antiviral activity found in cervicovaginal secretions is critical. Cervicovaginal lavage (CVL) samples were obtained from 20 healthy women and evaluated for anti-herpes simplex virus (HSV) activity. CVL samples reduced HSV-2 yields by 23-fold (median), and the anti-HSV activity of CVL samples correlated with the concentration of human neutrophil peptides (HNP)-1-3. Both CVL samples and HNP-1-3 interacted with virus and prevented entry after binding. Substantially less protective activity was observed in CVL samples obtained from 20 human immunodeficiency virus-infected subjects, but the addition of CVL samples from healthy subjects enhanced the antiviral activity. The significance of the innate activity was further demonstrated by showing that CVL samples prevented murine genital herpes. Fourteen of 15 mice were protected from genital herpes if they were challenged with HSV-2 pretreated with CVL samples from healthy subjects. In contrast, all 15 mice challenged with untreated HSV-2 died. These findings are evidence that cervicovaginal secretions contribute to innate resistance to HSV-2 and identify defensins as contributors to this activity.

UR - http://www.scopus.com/inward/record.url?scp=27744561668&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=27744561668&partnerID=8YFLogxK

U2 - 10.1086/497168

DO - 10.1086/497168

M3 - Article

C2 - 16235171

AN - SCOPUS:27744561668

VL - 192

SP - 1731

EP - 1740

JO - Journal of Infectious Diseases

JF - Journal of Infectious Diseases

SN - 0022-1899

IS - 10

ER -