Cervicovaginal secretions contribute to innate resistance to herpes simplex virus infection

Minnie John, Marla J. Keller, Ehsan H. Fam, Natalia Cheshenko, Kathleen Hogarty, Andrea Kosowitz, Sylvan Wallenstein, Maria J. Carlucci, Ana C. Tuyama, Wuyyan Lu, Mary E. Klotman, Robert I. Lehrer, Betsy C. Herold

Research output: Contribution to journalArticle

65 Scopus citations

Abstract

Defining and preserving the innate antiviral activity found in cervicovaginal secretions is critical. Cervicovaginal lavage (CVL) samples were obtained from 20 healthy women and evaluated for anti-herpes simplex virus (HSV) activity. CVL samples reduced HSV-2 yields by 23-fold (median), and the anti-HSV activity of CVL samples correlated with the concentration of human neutrophil peptides (HNP)-1-3. Both CVL samples and HNP-1-3 interacted with virus and prevented entry after binding. Substantially less protective activity was observed in CVL samples obtained from 20 human immunodeficiency virus-infected subjects, but the addition of CVL samples from healthy subjects enhanced the antiviral activity. The significance of the innate activity was further demonstrated by showing that CVL samples prevented murine genital herpes. Fourteen of 15 mice were protected from genital herpes if they were challenged with HSV-2 pretreated with CVL samples from healthy subjects. In contrast, all 15 mice challenged with untreated HSV-2 died. These findings are evidence that cervicovaginal secretions contribute to innate resistance to HSV-2 and identify defensins as contributors to this activity.

Original languageEnglish (US)
Pages (from-to)1731-1740
Number of pages10
JournalJournal of Infectious Diseases
Volume192
Issue number10
DOIs
StatePublished - Nov 15 2005
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

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  • Cite this

    John, M., Keller, M. J., Fam, E. H., Cheshenko, N., Hogarty, K., Kosowitz, A., Wallenstein, S., Carlucci, M. J., Tuyama, A. C., Lu, W., Klotman, M. E., Lehrer, R. I., & Herold, B. C. (2005). Cervicovaginal secretions contribute to innate resistance to herpes simplex virus infection. Journal of Infectious Diseases, 192(10), 1731-1740. https://doi.org/10.1086/497168