Cervical concentrations of interleukin-10 and interleukin-12 do not correlate with plasma levels

Philip E. Castle, Allan Hildesheim, Fredrick P. Bowman, Howard Strickler, Joan L. Walker, Terri Pustilnik, Robert P. Edwards, Peggy A. Crowley-Nowick

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Human papillomavirus (HPV) infects the transformation zone of the cervix and is the primary cause of cervical cancer. The infection is localized to the cervix and mucosal immunity is likely to be an important determinant for viral clearance. Previous studies of immunity to HPV have measured immune markers in the blood, but the relationship of systemic immunity to cervical immunity is poorly understood. In this study of 70 women enrolled in the ASCUS-LSIL Triage Study (ALTS), a clinical trial for management of low-grade cytologic abnormalities of the cervix, we collected paired plasma and cervical secretions to investigate the relationship between cervical concentrations of interleukin-10 (IL-10) and interleukin-12 (IL-12) and plasma levels. Neither IL-10 (ρ = 0.11), or IL-12 (ρ = -0.04) nor the ratio of IL-12 to IL-10 (ρ = 0.06) were correlated between blood and cervical secretions. Except for weak correlations of IL-10 among nonsmokers (ρ = 0.35, P = 0.019) and those in day 18-27 of their menstrual cycle (ρ = 0.51, P = 0.015), this lack of correlation persisted in all subgroups defined by genital inflammation or infection, current oral contraceptive use, heme contamination and volume of collected secretions, HPV16 seropositivity, and repeat HPV infection and/or cytologic abnormalities. The lack of correlation and high concentrations in cervical secretions indicate that the cervical IL-10 and IL-12 concentrations exceed what could be expected from blood as a principle source of IL-10 and IL-12 and suggest that cytokine concentrations in cervical secretions are predominantly the result of local cytokine production.

Original languageEnglish (US)
Pages (from-to)23-27
Number of pages5
JournalJournal of Clinical Immunology
Volume22
Issue number1
DOIs
StatePublished - 2002

Fingerprint

Interleukin-12
Interleukin-10
Cervix Uteri
Immunity
Cytokines
Mucosal Immunity
Papillomavirus Infections
Triage
Menstrual Cycle
Oral Contraceptives
Infection
Heme
Uterine Cervical Neoplasms
Biomarkers
Clinical Trials
Inflammation

Keywords

  • Cervix, plasma, HPV
  • IL-10
  • IL-12

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Cervical concentrations of interleukin-10 and interleukin-12 do not correlate with plasma levels. / Castle, Philip E.; Hildesheim, Allan; Bowman, Fredrick P.; Strickler, Howard; Walker, Joan L.; Pustilnik, Terri; Edwards, Robert P.; Crowley-Nowick, Peggy A.

In: Journal of Clinical Immunology, Vol. 22, No. 1, 2002, p. 23-27.

Research output: Contribution to journalArticle

Castle, PE, Hildesheim, A, Bowman, FP, Strickler, H, Walker, JL, Pustilnik, T, Edwards, RP & Crowley-Nowick, PA 2002, 'Cervical concentrations of interleukin-10 and interleukin-12 do not correlate with plasma levels', Journal of Clinical Immunology, vol. 22, no. 1, pp. 23-27. https://doi.org/10.1023/A:1014252402630
Castle, Philip E. ; Hildesheim, Allan ; Bowman, Fredrick P. ; Strickler, Howard ; Walker, Joan L. ; Pustilnik, Terri ; Edwards, Robert P. ; Crowley-Nowick, Peggy A. / Cervical concentrations of interleukin-10 and interleukin-12 do not correlate with plasma levels. In: Journal of Clinical Immunology. 2002 ; Vol. 22, No. 1. pp. 23-27.
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