Centrosomal localization of Diversin and its relevance to Wnt signaling

Keiji Itoh, Andreas Jenny, Marek Mlodzik, Sergei Y. Sokol

Research output: Contribution to journalArticle

31 Scopus citations

Abstract

Wnt pathways regulate many developmental processes, including cell-fate specification, cell polarity, and cell movements during morphogenesis. The subcellular distribution of pathway mediators in specific cellular compartments might be crucial for the selection of pathway targets and signaling specificity. We find that the ankyrin-repeat protein Diversin, which functions in different Wnt signaling branches, localizes to the centrosome in Xenopus ectoderm and mammalian cells. Upon stimulation with Wnt ligands, the centrosomal distribution of Diversin is transformed into punctate cortical localization. Also, Diversin was recruited by Frizzled receptors to nonhomogeneous Dishevelled-containing cortical patches. Importantly, Diversin deletion constructs, which did not localize to the centrosome, failed to efficiently antagonize Wnt signaling. Furthermore, a C-terminal construct that interfered with Diversin localization inhibited Diversin-mediated β-catenin degradation. These observations suggest that the centrosomal localization of Diversin is crucial for its function in Wnt signaling.

Original languageEnglish (US)
Pages (from-to)3791-3798
Number of pages8
JournalJournal of cell science
Volume122
Issue number20
DOIs
StatePublished - Oct 15 2009

Keywords

  • Cell polarity
  • Centrosome
  • Diversin
  • Frizzled
  • Wnt
  • Xenopus

ASJC Scopus subject areas

  • Cell Biology

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