Center for synchrotron biosciences' U2B beamline: An international resource for biological infrared spectroscopy

N. S. Marinkovic, R. Huang, P. Bromberg, M. Sullivan, J. Toomey, L. M. Miller, E. Sperber, Solomon L. Moshe, K. W. Jones, E. Chouparova, S. Lappi, S. Franzen, M. R. Chance

Research output: Contribution to journalArticle

76 Citations (Scopus)

Abstract

A synchrotron infrared (IR) beamline, U2B, dedicated to the biomedical and biological sciences has been constructed and is in operation at the National Synchrotron Light Source (NSLS) of Brookhaven National Laboratory. The facility is operated by the Center for Synchrotron Biosciences of the Albert Einstein College of Medicine in cooperation with the NSLS. Owing to the broadband nature of the synchrotron beam with brightness 1000 times that of conventional sources, Fourier transform IR spectroscopy experiments are feasible on diffraction-limited sample areas at high signalto-noise ratios and with relatively short data-acquisition times. A number of synchrotron IR microscopy experiments that have been performed in the mid-IR spectral range (500-5000cm-1) are summarized, including time-resolved protein-folding studies in the microsecond time regime, IR imaging of neurons, bone and other biological tissues, as well as imaging of samples of interest in the chemical and environmental sciences. Owing to the high flux output of this beamline in the far-IR region (50-500 cm-1), investigations of hydrogen bonding and dynamic molecular motions of biomolecules have been carried out from 10 to 300 K using a custom-made cryostat and an evacuated box. This facility is intended as an international resource for biological IR spectroscopy fully available to outside users based on competitive proposal.

Original languageEnglish (US)
Pages (from-to)189-197
Number of pages9
JournalJournal of Synchrotron Radiation
Volume9
Issue number4
DOIs
StatePublished - Jul 1 2002

Fingerprint

Synchrotrons
Infrared spectroscopy
resources
synchrotrons
infrared spectroscopy
Infrared radiation
Light sources
light sources
Protein folding
Cryostats
Infrared imaging
Biomolecules
cryostats
neurons
medicine
folding
bones
Neurons
Medicine
data acquisition

Keywords

  • Biological imaging
  • Bone
  • Infrared spectroscopy
  • Microscopy
  • Nucleic acid dynamics
  • Protein folding

ASJC Scopus subject areas

  • Instrumentation
  • Atomic and Molecular Physics, and Optics
  • Radiation
  • Physics and Astronomy (miscellaneous)

Cite this

Marinkovic, N. S., Huang, R., Bromberg, P., Sullivan, M., Toomey, J., Miller, L. M., ... Chance, M. R. (2002). Center for synchrotron biosciences' U2B beamline: An international resource for biological infrared spectroscopy. Journal of Synchrotron Radiation, 9(4), 189-197. https://doi.org/10.1107/S0909049502008543

Center for synchrotron biosciences' U2B beamline : An international resource for biological infrared spectroscopy. / Marinkovic, N. S.; Huang, R.; Bromberg, P.; Sullivan, M.; Toomey, J.; Miller, L. M.; Sperber, E.; Moshe, Solomon L.; Jones, K. W.; Chouparova, E.; Lappi, S.; Franzen, S.; Chance, M. R.

In: Journal of Synchrotron Radiation, Vol. 9, No. 4, 01.07.2002, p. 189-197.

Research output: Contribution to journalArticle

Marinkovic, NS, Huang, R, Bromberg, P, Sullivan, M, Toomey, J, Miller, LM, Sperber, E, Moshe, SL, Jones, KW, Chouparova, E, Lappi, S, Franzen, S & Chance, MR 2002, 'Center for synchrotron biosciences' U2B beamline: An international resource for biological infrared spectroscopy', Journal of Synchrotron Radiation, vol. 9, no. 4, pp. 189-197. https://doi.org/10.1107/S0909049502008543
Marinkovic, N. S. ; Huang, R. ; Bromberg, P. ; Sullivan, M. ; Toomey, J. ; Miller, L. M. ; Sperber, E. ; Moshe, Solomon L. ; Jones, K. W. ; Chouparova, E. ; Lappi, S. ; Franzen, S. ; Chance, M. R. / Center for synchrotron biosciences' U2B beamline : An international resource for biological infrared spectroscopy. In: Journal of Synchrotron Radiation. 2002 ; Vol. 9, No. 4. pp. 189-197.
@article{1bb5d818bb73461f8b523d6f2162fd1d,
title = "Center for synchrotron biosciences' U2B beamline: An international resource for biological infrared spectroscopy",
abstract = "A synchrotron infrared (IR) beamline, U2B, dedicated to the biomedical and biological sciences has been constructed and is in operation at the National Synchrotron Light Source (NSLS) of Brookhaven National Laboratory. The facility is operated by the Center for Synchrotron Biosciences of the Albert Einstein College of Medicine in cooperation with the NSLS. Owing to the broadband nature of the synchrotron beam with brightness 1000 times that of conventional sources, Fourier transform IR spectroscopy experiments are feasible on diffraction-limited sample areas at high signalto-noise ratios and with relatively short data-acquisition times. A number of synchrotron IR microscopy experiments that have been performed in the mid-IR spectral range (500-5000cm-1) are summarized, including time-resolved protein-folding studies in the microsecond time regime, IR imaging of neurons, bone and other biological tissues, as well as imaging of samples of interest in the chemical and environmental sciences. Owing to the high flux output of this beamline in the far-IR region (50-500 cm-1), investigations of hydrogen bonding and dynamic molecular motions of biomolecules have been carried out from 10 to 300 K using a custom-made cryostat and an evacuated box. This facility is intended as an international resource for biological IR spectroscopy fully available to outside users based on competitive proposal.",
keywords = "Biological imaging, Bone, Infrared spectroscopy, Microscopy, Nucleic acid dynamics, Protein folding",
author = "Marinkovic, {N. S.} and R. Huang and P. Bromberg and M. Sullivan and J. Toomey and Miller, {L. M.} and E. Sperber and Moshe, {Solomon L.} and Jones, {K. W.} and E. Chouparova and S. Lappi and S. Franzen and Chance, {M. R.}",
year = "2002",
month = "7",
day = "1",
doi = "10.1107/S0909049502008543",
language = "English (US)",
volume = "9",
pages = "189--197",
journal = "Journal of Synchrotron Radiation",
issn = "0909-0495",
publisher = "International Union of Crystallography",
number = "4",

}

TY - JOUR

T1 - Center for synchrotron biosciences' U2B beamline

T2 - An international resource for biological infrared spectroscopy

AU - Marinkovic, N. S.

AU - Huang, R.

AU - Bromberg, P.

AU - Sullivan, M.

AU - Toomey, J.

AU - Miller, L. M.

AU - Sperber, E.

AU - Moshe, Solomon L.

AU - Jones, K. W.

AU - Chouparova, E.

AU - Lappi, S.

AU - Franzen, S.

AU - Chance, M. R.

PY - 2002/7/1

Y1 - 2002/7/1

N2 - A synchrotron infrared (IR) beamline, U2B, dedicated to the biomedical and biological sciences has been constructed and is in operation at the National Synchrotron Light Source (NSLS) of Brookhaven National Laboratory. The facility is operated by the Center for Synchrotron Biosciences of the Albert Einstein College of Medicine in cooperation with the NSLS. Owing to the broadband nature of the synchrotron beam with brightness 1000 times that of conventional sources, Fourier transform IR spectroscopy experiments are feasible on diffraction-limited sample areas at high signalto-noise ratios and with relatively short data-acquisition times. A number of synchrotron IR microscopy experiments that have been performed in the mid-IR spectral range (500-5000cm-1) are summarized, including time-resolved protein-folding studies in the microsecond time regime, IR imaging of neurons, bone and other biological tissues, as well as imaging of samples of interest in the chemical and environmental sciences. Owing to the high flux output of this beamline in the far-IR region (50-500 cm-1), investigations of hydrogen bonding and dynamic molecular motions of biomolecules have been carried out from 10 to 300 K using a custom-made cryostat and an evacuated box. This facility is intended as an international resource for biological IR spectroscopy fully available to outside users based on competitive proposal.

AB - A synchrotron infrared (IR) beamline, U2B, dedicated to the biomedical and biological sciences has been constructed and is in operation at the National Synchrotron Light Source (NSLS) of Brookhaven National Laboratory. The facility is operated by the Center for Synchrotron Biosciences of the Albert Einstein College of Medicine in cooperation with the NSLS. Owing to the broadband nature of the synchrotron beam with brightness 1000 times that of conventional sources, Fourier transform IR spectroscopy experiments are feasible on diffraction-limited sample areas at high signalto-noise ratios and with relatively short data-acquisition times. A number of synchrotron IR microscopy experiments that have been performed in the mid-IR spectral range (500-5000cm-1) are summarized, including time-resolved protein-folding studies in the microsecond time regime, IR imaging of neurons, bone and other biological tissues, as well as imaging of samples of interest in the chemical and environmental sciences. Owing to the high flux output of this beamline in the far-IR region (50-500 cm-1), investigations of hydrogen bonding and dynamic molecular motions of biomolecules have been carried out from 10 to 300 K using a custom-made cryostat and an evacuated box. This facility is intended as an international resource for biological IR spectroscopy fully available to outside users based on competitive proposal.

KW - Biological imaging

KW - Bone

KW - Infrared spectroscopy

KW - Microscopy

KW - Nucleic acid dynamics

KW - Protein folding

UR - http://www.scopus.com/inward/record.url?scp=0036657553&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036657553&partnerID=8YFLogxK

U2 - 10.1107/S0909049502008543

DO - 10.1107/S0909049502008543

M3 - Article

C2 - 12091724

AN - SCOPUS:0036657553

VL - 9

SP - 189

EP - 197

JO - Journal of Synchrotron Radiation

JF - Journal of Synchrotron Radiation

SN - 0909-0495

IS - 4

ER -