Cellular requirements for lipopolysaccharide adjuvanticity. A role for both T lymphocytes and macrophages for in vitro responses to particulate antigens

J. R. McGhee, J. J. Farrar, S. M. Michalek, S. E. Mergenhagen, David L. Rosenstreich

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

By employing primary cultures of purified spleen cells from lipopolysaccaride (LPS) responder (C3H/HeN or C57BL/10Sn) or nonresponder (C3H/HeJ or C57BL/10ScN) mice incubated with particulate antigen and LPS prepared by phenol-water extraction (Ph), we have presented evidence that both T cells and macrophages (M∅) are required for LPS-induced adjuvanticity. First, M∅ derived from C3H/HeN spleen cells, when mixed with responder, C3H/HeN lymphoctes and Ph-LPS, elicited enhanced antibody responses to sheep erthrocyte (SRC) antigen, whereas, lymphocytes from the nonresponder, C3H/HeJ mouse strain did not evoke this response. Similarly, purified T cells from C3H/HeN spleens, when cultured with responder, nu/nu spleen cells, and Ph/LPS yielded enhanced anti-TNP PFC responses; whereas, C3H/HeJ T cells did not potentiate immune responses when mixed with optimal concentrations of Ph-LPS. LPS prepared by butanol-water extraction elicited significant adjuvant effects with all cell combinations. Finally, purified responder T cells and M∅ enabled either responder or nonresponder B cells to elicit LPS potentiation. These data indicate that T cells and M∅ are controlling LPS-induced augmentation of B-cell responses.

Original languageEnglish (US)
Pages (from-to)793-807
Number of pages15
JournalJournal of Experimental Medicine
Volume149
Issue number4
DOIs
StatePublished - 1979
Externally publishedYes

Fingerprint

Lipopolysaccharides
Macrophages
T-Lymphocytes
Antigens
Spleen
B-Lymphocytes
Butanols
Water
Inbred C3H Mouse
Phenol
Inbred C57BL Mouse
Antibody Formation
Sheep
In Vitro Techniques
Lymphocytes

ASJC Scopus subject areas

  • Immunology

Cite this

Cellular requirements for lipopolysaccharide adjuvanticity. A role for both T lymphocytes and macrophages for in vitro responses to particulate antigens. / McGhee, J. R.; Farrar, J. J.; Michalek, S. M.; Mergenhagen, S. E.; Rosenstreich, David L.

In: Journal of Experimental Medicine, Vol. 149, No. 4, 1979, p. 793-807.

Research output: Contribution to journalArticle

@article{3104250b682c4b8cb85b34458cd14ee9,
title = "Cellular requirements for lipopolysaccharide adjuvanticity. A role for both T lymphocytes and macrophages for in vitro responses to particulate antigens",
abstract = "By employing primary cultures of purified spleen cells from lipopolysaccaride (LPS) responder (C3H/HeN or C57BL/10Sn) or nonresponder (C3H/HeJ or C57BL/10ScN) mice incubated with particulate antigen and LPS prepared by phenol-water extraction (Ph), we have presented evidence that both T cells and macrophages (M∅) are required for LPS-induced adjuvanticity. First, M∅ derived from C3H/HeN spleen cells, when mixed with responder, C3H/HeN lymphoctes and Ph-LPS, elicited enhanced antibody responses to sheep erthrocyte (SRC) antigen, whereas, lymphocytes from the nonresponder, C3H/HeJ mouse strain did not evoke this response. Similarly, purified T cells from C3H/HeN spleens, when cultured with responder, nu/nu spleen cells, and Ph/LPS yielded enhanced anti-TNP PFC responses; whereas, C3H/HeJ T cells did not potentiate immune responses when mixed with optimal concentrations of Ph-LPS. LPS prepared by butanol-water extraction elicited significant adjuvant effects with all cell combinations. Finally, purified responder T cells and M∅ enabled either responder or nonresponder B cells to elicit LPS potentiation. These data indicate that T cells and M∅ are controlling LPS-induced augmentation of B-cell responses.",
author = "McGhee, {J. R.} and Farrar, {J. J.} and Michalek, {S. M.} and Mergenhagen, {S. E.} and Rosenstreich, {David L.}",
year = "1979",
doi = "10.1084/jem.149.4.793",
language = "English (US)",
volume = "149",
pages = "793--807",
journal = "Journal of Experimental Medicine",
issn = "0022-1007",
publisher = "Rockefeller University Press",
number = "4",

}

TY - JOUR

T1 - Cellular requirements for lipopolysaccharide adjuvanticity. A role for both T lymphocytes and macrophages for in vitro responses to particulate antigens

AU - McGhee, J. R.

AU - Farrar, J. J.

AU - Michalek, S. M.

AU - Mergenhagen, S. E.

AU - Rosenstreich, David L.

PY - 1979

Y1 - 1979

N2 - By employing primary cultures of purified spleen cells from lipopolysaccaride (LPS) responder (C3H/HeN or C57BL/10Sn) or nonresponder (C3H/HeJ or C57BL/10ScN) mice incubated with particulate antigen and LPS prepared by phenol-water extraction (Ph), we have presented evidence that both T cells and macrophages (M∅) are required for LPS-induced adjuvanticity. First, M∅ derived from C3H/HeN spleen cells, when mixed with responder, C3H/HeN lymphoctes and Ph-LPS, elicited enhanced antibody responses to sheep erthrocyte (SRC) antigen, whereas, lymphocytes from the nonresponder, C3H/HeJ mouse strain did not evoke this response. Similarly, purified T cells from C3H/HeN spleens, when cultured with responder, nu/nu spleen cells, and Ph/LPS yielded enhanced anti-TNP PFC responses; whereas, C3H/HeJ T cells did not potentiate immune responses when mixed with optimal concentrations of Ph-LPS. LPS prepared by butanol-water extraction elicited significant adjuvant effects with all cell combinations. Finally, purified responder T cells and M∅ enabled either responder or nonresponder B cells to elicit LPS potentiation. These data indicate that T cells and M∅ are controlling LPS-induced augmentation of B-cell responses.

AB - By employing primary cultures of purified spleen cells from lipopolysaccaride (LPS) responder (C3H/HeN or C57BL/10Sn) or nonresponder (C3H/HeJ or C57BL/10ScN) mice incubated with particulate antigen and LPS prepared by phenol-water extraction (Ph), we have presented evidence that both T cells and macrophages (M∅) are required for LPS-induced adjuvanticity. First, M∅ derived from C3H/HeN spleen cells, when mixed with responder, C3H/HeN lymphoctes and Ph-LPS, elicited enhanced antibody responses to sheep erthrocyte (SRC) antigen, whereas, lymphocytes from the nonresponder, C3H/HeJ mouse strain did not evoke this response. Similarly, purified T cells from C3H/HeN spleens, when cultured with responder, nu/nu spleen cells, and Ph/LPS yielded enhanced anti-TNP PFC responses; whereas, C3H/HeJ T cells did not potentiate immune responses when mixed with optimal concentrations of Ph-LPS. LPS prepared by butanol-water extraction elicited significant adjuvant effects with all cell combinations. Finally, purified responder T cells and M∅ enabled either responder or nonresponder B cells to elicit LPS potentiation. These data indicate that T cells and M∅ are controlling LPS-induced augmentation of B-cell responses.

UR - http://www.scopus.com/inward/record.url?scp=0018343814&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0018343814&partnerID=8YFLogxK

U2 - 10.1084/jem.149.4.793

DO - 10.1084/jem.149.4.793

M3 - Article

C2 - 372482

AN - SCOPUS:0018343814

VL - 149

SP - 793

EP - 807

JO - Journal of Experimental Medicine

JF - Journal of Experimental Medicine

SN - 0022-1007

IS - 4

ER -