Cellular expression of the gut chitinase in the stomach of frogs Xenopus laevis and Rana catesbeiana

Wakako Fujimoto, Masako Suzuki, Kazuhiro Kimura, Toshihiko Iwanaga

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Gut chitinase, originally identified as acidic mammalian chitinase, is secreted from the salivary gland and stomach in humans and mice, but exclusively from the liver in cattle. Since the organs producing gut chitinase differ depending on the species, here we determined the expression site of this enzyme in the gastrointestinal tract of frogs, Xenopus laevis and Rana catesbeiana, which eat a large amount of chitin-coated animals, using in situ hybridization and immunohistochemistry. The chitinase was detected only in the oxynticopeptic cells of gastric glands and was accumulated in secretory granules in the cytoplasm, suggesting that the enzyme may be released into the gastric lumen directly. In addition, the enzyme was not found in the stomach of larvae, but was detected at stage 62 of metamorphosis and later, when the frogs changed from a herbivorous to a carnivorous diet.

Original languageEnglish (US)
Pages (from-to)91-99
Number of pages9
JournalBiomedical Research
Volume23
Issue number2
StatePublished - 2002
Externally publishedYes

Fingerprint

Rana catesbeiana
Chitinases
Xenopus laevis
Anura
Stomach
Enzymes
Chitin
Secretory Vesicles
Nutrition
Gastric Mucosa
Salivary Glands
Liver
In Situ Hybridization
Larva
Gastrointestinal Tract
Cytoplasm
Animals
Immunohistochemistry
Diet

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Cellular expression of the gut chitinase in the stomach of frogs Xenopus laevis and Rana catesbeiana. / Fujimoto, Wakako; Suzuki, Masako; Kimura, Kazuhiro; Iwanaga, Toshihiko.

In: Biomedical Research, Vol. 23, No. 2, 2002, p. 91-99.

Research output: Contribution to journalArticle

Fujimoto, Wakako ; Suzuki, Masako ; Kimura, Kazuhiro ; Iwanaga, Toshihiko. / Cellular expression of the gut chitinase in the stomach of frogs Xenopus laevis and Rana catesbeiana. In: Biomedical Research. 2002 ; Vol. 23, No. 2. pp. 91-99.
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