Cellular and molecular changes in 3T3 cells transformed spontaneously or by DNA transfection

M. Z. Wahrman, S. E. Gagnier, D. R. Kobrin, P. J. Higgins, Leonard H. Augenlicht

Research output: Contribution to journalArticle

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Abstract

Transformed cell lines have been selected following exposure of NIH 3T3 cells to a calcium phosphate precipitate containing DNA from: the human colon carcinoma cell line, SW 480; the cloned Harvey sarcoma virus ras gene; the parental NIH 3T3 cell line; or no DNA (a spontaneous transformant). Unlike the parental 3T3 cells, each of these lines readily formed malignant spindle cell tumors in Swiss nu/nu mice. Southern blots confirmed the presence of the human homologue of the Kirsten ras gene in the cells transformed by SW480 DNA, and the Harvey ras gene in the cells transformed with that cloned sequence. The morphology of each of the lines was different, the cells transformed with the human and viral ras genes being the most aberrant (but not identical) and forming the most extensive foci in culture. These ras transformed lines also exhibited anchorage-independent growth, while the two other transformed lines did not. Both of the ras transformed lines, as well as the spontaneously transformed line, exhibited a pronounced disruption of actin microfilament structure. Two-dimensional gel electrophoresis of 35S-methionine-labeled peptides revealed that these three lines also had a marked decrease in an acidic peptide of 32K daltons.

Original languageEnglish (US)
Pages (from-to)41-56
Number of pages16
JournalTumour Biology
Volume6
Issue number1
StatePublished - 1985
Externally publishedYes

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3T3 Cells
ras Genes
Transfection
NIH 3T3 Cells
DNA
Cell Line
Harvey murine sarcoma virus
Peptides
Transformed Cell Line
Viral Genes
Electrophoresis, Gel, Two-Dimensional
Southern Blotting
Actin Cytoskeleton
Methionine
Colon
Carcinoma
Growth
Neoplasms

ASJC Scopus subject areas

  • Cancer Research

Cite this

Wahrman, M. Z., Gagnier, S. E., Kobrin, D. R., Higgins, P. J., & Augenlicht, L. H. (1985). Cellular and molecular changes in 3T3 cells transformed spontaneously or by DNA transfection. Tumour Biology, 6(1), 41-56.

Cellular and molecular changes in 3T3 cells transformed spontaneously or by DNA transfection. / Wahrman, M. Z.; Gagnier, S. E.; Kobrin, D. R.; Higgins, P. J.; Augenlicht, Leonard H.

In: Tumour Biology, Vol. 6, No. 1, 1985, p. 41-56.

Research output: Contribution to journalArticle

Wahrman, MZ, Gagnier, SE, Kobrin, DR, Higgins, PJ & Augenlicht, LH 1985, 'Cellular and molecular changes in 3T3 cells transformed spontaneously or by DNA transfection', Tumour Biology, vol. 6, no. 1, pp. 41-56.
Wahrman, M. Z. ; Gagnier, S. E. ; Kobrin, D. R. ; Higgins, P. J. ; Augenlicht, Leonard H. / Cellular and molecular changes in 3T3 cells transformed spontaneously or by DNA transfection. In: Tumour Biology. 1985 ; Vol. 6, No. 1. pp. 41-56.
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