TY - JOUR
T1 - Cellular and Extracellular Vesicle RNA Analysis in the Global Threat Fungus Candida auris
AU - Munhoz da Rocha, Isadora F.
AU - Martins, Sharon T.
AU - Amatuzzi, Rafaela F.
AU - Zamith-Miranda, Daniel
AU - Nosanchuk, Joshua D.
AU - Rodrigues, Marcio L.
AU - Alves, Lysangela R.
N1 - Funding Information:
J.D.N. and D.Z-M. were partially supported by NIH R21 AI124797. M.L.R. was supported by grants from the Brazilian Ministry of Health (grant no. 440015/2018-9), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, grant no. 405520/2018-2 and no. 301304/ 2017-3) and Fiocruz (grant no. VPPCB-007-FIO-18 and no. VPPIS-001-FIO18). The authors also acknowledge support from the Instituto Nacional de Ciência e Tecnologia de Inovação em Doenças de Populações Negligenciadas (INCT-IDPN). M.L.R. is currently on leave from the position of Associate Professor at the Microbiology Institute of the Federal University of Rio de Janeiro, Brazil. L.R.A. received financial support from Inova Fiocruz/Fundação Oswaldo Cruz (grant no. VPPCB-07-FIO-18-2-52) and CNPq (grant no. 442317/2019-0). L.R.A is a research fellow awardee from CNPq.
Publisher Copyright:
Copyright © 2021 Munhoz da Rocha et al.
PY - 2021/12
Y1 - 2021/12
N2 - Emerging and reemerging pathogens are a worldwide concern, and it is predicted that these microbes will cause severe outbreaks. Candida auris affects people with weakened immune systems, particularly those who are hospitalized or are in health care facilities. Extracellular vesicles (EVs) are lipid bilayer structures released by organisms from all domains of life. EVs can deliver functional molecules to target cells, including proteins and nucleic acids, especially RNA molecules. EVs from several pathogenic fungi species play diverse biological roles related to cell-cell communication and pathogen-host interaction. In this study, we describe a data set which we produced by sequencing the RNA content of EVs from C. auris under normal growth conditions and in the presence of the antifungal caspofungin, a first-line drug to treat this fungus. To generate a more complete data set for future comparative studies, we also sequenced the RNA cellular content of EVs under the same conditions. This data set addresses a previously unexplored area of fungal biology regarding cellular small RNA and EV RNA. Our data will provide a molecular basis for the study of the aspects associated with antifungal treatment, gene expression response, and EV composition in C. auris. These data will also allow the exploration of small RNA content in the fungal kingdom and might serve as an informative basis for studies on the mechanisms by which molecules are directed to fungal EVs. IMPORTANCE Candida auris, a relevant emerging human-pathogenic yeast, is the first fungus to be called a global public health threat by the WHO. This is because of its rapid spread on all inhabited continents, together with its extremely high frequency of drug and multidrug resistance. In our study, we generated a large data set for 3 distinct strains of C. auris and obtained cellular small RNA fraction as well as extracellular vesicle RNA (EV-RNA) during normal growth conditions and after treatment with caspofungin, the first-line drug used to treat C. auris infection.
AB - Emerging and reemerging pathogens are a worldwide concern, and it is predicted that these microbes will cause severe outbreaks. Candida auris affects people with weakened immune systems, particularly those who are hospitalized or are in health care facilities. Extracellular vesicles (EVs) are lipid bilayer structures released by organisms from all domains of life. EVs can deliver functional molecules to target cells, including proteins and nucleic acids, especially RNA molecules. EVs from several pathogenic fungi species play diverse biological roles related to cell-cell communication and pathogen-host interaction. In this study, we describe a data set which we produced by sequencing the RNA content of EVs from C. auris under normal growth conditions and in the presence of the antifungal caspofungin, a first-line drug to treat this fungus. To generate a more complete data set for future comparative studies, we also sequenced the RNA cellular content of EVs under the same conditions. This data set addresses a previously unexplored area of fungal biology regarding cellular small RNA and EV RNA. Our data will provide a molecular basis for the study of the aspects associated with antifungal treatment, gene expression response, and EV composition in C. auris. These data will also allow the exploration of small RNA content in the fungal kingdom and might serve as an informative basis for studies on the mechanisms by which molecules are directed to fungal EVs. IMPORTANCE Candida auris, a relevant emerging human-pathogenic yeast, is the first fungus to be called a global public health threat by the WHO. This is because of its rapid spread on all inhabited continents, together with its extremely high frequency of drug and multidrug resistance. In our study, we generated a large data set for 3 distinct strains of C. auris and obtained cellular small RNA fraction as well as extracellular vesicle RNA (EV-RNA) during normal growth conditions and after treatment with caspofungin, the first-line drug used to treat C. auris infection.
KW - Candida auris
KW - Dataset
KW - Extracellular vesicles
KW - RNA-seq
KW - Small RNA
UR - http://www.scopus.com/inward/record.url?scp=85122802999&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85122802999&partnerID=8YFLogxK
U2 - 10.1128/SPECTRUM.01538-21
DO - 10.1128/SPECTRUM.01538-21
M3 - Article
C2 - 34908466
AN - SCOPUS:85122802999
SN - 2165-0497
VL - 9
JO - Microbiology spectrum
JF - Microbiology spectrum
IS - 3
M1 - e01538
ER -
