Cell surface expression of a specific antigenic site on the catalytic subunit of (Na+ + K+)-ATPase

Allen C. Myers, Bruce S. Bochner, Gordon F. Tomaselli, Neal Fedarko, Sherry A. Hudson, Holly Rohde, Shau Ku Huang, Kai Y. Xu

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Structural localization of a peptide region, KRQPRNPKTDKLVNE, in the catalytic subunit of (Na+ + K+)-ATPase was investigated using a specific antibody directed against this peptide in cultured African green monkey kidney CV-1 cells. Immunofluorescence staining of frozen cell sections shows that an anti-KRQPRNPKTDKLVNE antibody (SSA95) interacts with its antigenic site and binds to the extracellular side of the cell membrane. Indirect immunofluorescence and flow cytometric analyses confirmed the presence of this epitope on intact cell surfaces. These results suggest that the KRQPRNPKTDKLVNE region of the (Na+ + K+)-ATPase is expressed on the cellular membrane surface.

Original languageEnglish (US)
Pages (from-to)111-115
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume291
Issue number1
DOIs
StatePublished - Jan 1 2002
Externally publishedYes

Fingerprint

Adenosine Triphosphatases
Catalytic Domain
Peptides
Cell membranes
Cercopithecus aethiops
Epitopes
Anti-Idiotypic Antibodies
Frozen Sections
Indirect Fluorescent Antibody Technique
Membranes
Fluorescent Antibody Technique
Antibodies
Cell Membrane
Staining and Labeling
Kidney
sodium-translocating ATPase
1,2-diamino-1,2-N,N'-carbonyl-1,2-dideoxyglucose hydrate

Keywords

  • (Na + K)-ATPase
  • Flow cytometry
  • Immunofluorescence staining
  • Positive inotropy
  • Site-specific antibody

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Cell surface expression of a specific antigenic site on the catalytic subunit of (Na+ + K+)-ATPase. / Myers, Allen C.; Bochner, Bruce S.; Tomaselli, Gordon F.; Fedarko, Neal; Hudson, Sherry A.; Rohde, Holly; Huang, Shau Ku; Xu, Kai Y.

In: Biochemical and Biophysical Research Communications, Vol. 291, No. 1, 01.01.2002, p. 111-115.

Research output: Contribution to journalArticle

Myers, Allen C. ; Bochner, Bruce S. ; Tomaselli, Gordon F. ; Fedarko, Neal ; Hudson, Sherry A. ; Rohde, Holly ; Huang, Shau Ku ; Xu, Kai Y. / Cell surface expression of a specific antigenic site on the catalytic subunit of (Na+ + K+)-ATPase. In: Biochemical and Biophysical Research Communications. 2002 ; Vol. 291, No. 1. pp. 111-115.
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AU - Hudson, Sherry A.

AU - Rohde, Holly

AU - Huang, Shau Ku

AU - Xu, Kai Y.

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