Abstract
Elevated interstitial fluid pressure (IFP) is observed in most solid tumors. However, the study of the cellular processes of tumors and the development of chemotherapy are routinely studied using in vitro culture systems at atmospheric pressure. Using a new pressurized cell culture system, we investigated the influence of hydrostatic pressure on population dynamics of three primary osteosarcoma (HOS, U2OS, SaOS2) and two metastatic tumor cell lines (MCF7 breast, H1299 lung) that invade bone. Values of IFP in normal human bone and muscle, and in osteosarcoma tumors obtained during their surgical biopsy established the hydrostatic pressure range for the in vitro cell studies. The IFP values were obtained from a retrospective review of patient records. IFP from confirmed osteosarcoma was 35.9± 16.2 mmHg. Tumor IFP was significantly higher than muscle IFP (p < 0.001) and bone IFP (p < 0.003). The in vitro study measured the cell-line proliferation using hydrostatic pressures of 0, 20, 50 and 100 mmHg. The findings suggest that hydrostatic pressure either increases or decreases tumor proliferation rates depending on cell type. Furthermore, cell death was not associated with apoptosis.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1270-1280 |
| Number of pages | 11 |
| Journal | Annals of Biomedical Engineering |
| Volume | 33 |
| Issue number | 9 |
| DOIs | |
| State | Published - Sep 2005 |
| Externally published | Yes |
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Keywords
- Breast cancer
- Cell culture
- Interstitial fluid pressure
- Logistic equation
- Lung cancer
- Osteosarcoma
- Population dynamics
ASJC Scopus subject areas
- Biomedical Engineering
Cite this
Cell proliferation of cultured human cancer cells are affected by the elevated tumor pressures that exist in vivo. / DiResta, Gene R.; Nathan, Saminathan S.; Manoso, Mark W.; Casas-Ganem, Jorge; Wyatt, Chris; Kubo, Tadaheko; Boland, Patrick J.; Athanasian, Edward A.; Miodownik, Jonathan; Gorlick, Richard; Healey, John H.
In: Annals of Biomedical Engineering, Vol. 33, No. 9, 09.2005, p. 1270-1280.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Cell proliferation of cultured human cancer cells are affected by the elevated tumor pressures that exist in vivo
AU - DiResta, Gene R.
AU - Nathan, Saminathan S.
AU - Manoso, Mark W.
AU - Casas-Ganem, Jorge
AU - Wyatt, Chris
AU - Kubo, Tadaheko
AU - Boland, Patrick J.
AU - Athanasian, Edward A.
AU - Miodownik, Jonathan
AU - Gorlick, Richard
AU - Healey, John H.
PY - 2005/9
Y1 - 2005/9
N2 - Elevated interstitial fluid pressure (IFP) is observed in most solid tumors. However, the study of the cellular processes of tumors and the development of chemotherapy are routinely studied using in vitro culture systems at atmospheric pressure. Using a new pressurized cell culture system, we investigated the influence of hydrostatic pressure on population dynamics of three primary osteosarcoma (HOS, U2OS, SaOS2) and two metastatic tumor cell lines (MCF7 breast, H1299 lung) that invade bone. Values of IFP in normal human bone and muscle, and in osteosarcoma tumors obtained during their surgical biopsy established the hydrostatic pressure range for the in vitro cell studies. The IFP values were obtained from a retrospective review of patient records. IFP from confirmed osteosarcoma was 35.9± 16.2 mmHg. Tumor IFP was significantly higher than muscle IFP (p < 0.001) and bone IFP (p < 0.003). The in vitro study measured the cell-line proliferation using hydrostatic pressures of 0, 20, 50 and 100 mmHg. The findings suggest that hydrostatic pressure either increases or decreases tumor proliferation rates depending on cell type. Furthermore, cell death was not associated with apoptosis.
AB - Elevated interstitial fluid pressure (IFP) is observed in most solid tumors. However, the study of the cellular processes of tumors and the development of chemotherapy are routinely studied using in vitro culture systems at atmospheric pressure. Using a new pressurized cell culture system, we investigated the influence of hydrostatic pressure on population dynamics of three primary osteosarcoma (HOS, U2OS, SaOS2) and two metastatic tumor cell lines (MCF7 breast, H1299 lung) that invade bone. Values of IFP in normal human bone and muscle, and in osteosarcoma tumors obtained during their surgical biopsy established the hydrostatic pressure range for the in vitro cell studies. The IFP values were obtained from a retrospective review of patient records. IFP from confirmed osteosarcoma was 35.9± 16.2 mmHg. Tumor IFP was significantly higher than muscle IFP (p < 0.001) and bone IFP (p < 0.003). The in vitro study measured the cell-line proliferation using hydrostatic pressures of 0, 20, 50 and 100 mmHg. The findings suggest that hydrostatic pressure either increases or decreases tumor proliferation rates depending on cell type. Furthermore, cell death was not associated with apoptosis.
KW - Breast cancer
KW - Cell culture
KW - Interstitial fluid pressure
KW - Logistic equation
KW - Lung cancer
KW - Osteosarcoma
KW - Population dynamics
UR - http://www.scopus.com/inward/record.url?scp=24144461330&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=24144461330&partnerID=8YFLogxK
U2 - 10.1007/s10439-005-5732-9
DO - 10.1007/s10439-005-5732-9
M3 - Article
C2 - 16133932
AN - SCOPUS:24144461330
VL - 33
SP - 1270
EP - 1280
JO - Annals of Biomedical Engineering
JF - Annals of Biomedical Engineering
SN - 0090-6964
IS - 9
ER -