Cell proliferation in explant cultures of human colon

M. Usugane, M. Fujita, M. Lipkin, R. Palmer, E. Friedman, Leonard H. Augenlicht

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Biopsy specimens of human colonic mucosa taken from the rectosigmoid of 12 normal subjects were maintained in explant culture for 4 days. Histological, microautoradiographic and chemical measurements were carried out to evaluate cell replication, the effect of deoxycholic acid, and the incorporation of uridine and leucine into RNA and protein. Active cell replication was shown to be greatest during the first day of organ culture, and the number of cells that synthesized DNA also increased when deoxycholic acid was added to the culture medium. At later times, with morphological evidence of tissue viability, the synthesis of DNA, RNA, and protein within colonic epithelial cells decreased, and the total number of cells in the crypt columns declined. Findings indicate good maintenance of metabolic activities of colonic epithelial cells in short-term explant culture, and the utility of both cell-kinetic and morphological observations in assessing the status of colonic explants at early and late intervals.

Original languageEnglish (US)
Pages (from-to)225-233
Number of pages9
JournalDigestion
Volume24
Issue number4
StatePublished - 1982
Externally publishedYes

Fingerprint

Colon
Deoxycholic Acid
Cell Proliferation
Cell Count
Epithelial Cells
RNA
Tissue Survival
Uridine
Organ Culture Techniques
DNA
Leucine
Culture Media
Mucous Membrane
Proteins
Maintenance
Biopsy

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Usugane, M., Fujita, M., Lipkin, M., Palmer, R., Friedman, E., & Augenlicht, L. H. (1982). Cell proliferation in explant cultures of human colon. Digestion, 24(4), 225-233.

Cell proliferation in explant cultures of human colon. / Usugane, M.; Fujita, M.; Lipkin, M.; Palmer, R.; Friedman, E.; Augenlicht, Leonard H.

In: Digestion, Vol. 24, No. 4, 1982, p. 225-233.

Research output: Contribution to journalArticle

Usugane, M, Fujita, M, Lipkin, M, Palmer, R, Friedman, E & Augenlicht, LH 1982, 'Cell proliferation in explant cultures of human colon', Digestion, vol. 24, no. 4, pp. 225-233.
Usugane M, Fujita M, Lipkin M, Palmer R, Friedman E, Augenlicht LH. Cell proliferation in explant cultures of human colon. Digestion. 1982;24(4):225-233.
Usugane, M. ; Fujita, M. ; Lipkin, M. ; Palmer, R. ; Friedman, E. ; Augenlicht, Leonard H. / Cell proliferation in explant cultures of human colon. In: Digestion. 1982 ; Vol. 24, No. 4. pp. 225-233.
@article{11dcaa91c64748d49f0e2ea3a336b704,
title = "Cell proliferation in explant cultures of human colon",
abstract = "Biopsy specimens of human colonic mucosa taken from the rectosigmoid of 12 normal subjects were maintained in explant culture for 4 days. Histological, microautoradiographic and chemical measurements were carried out to evaluate cell replication, the effect of deoxycholic acid, and the incorporation of uridine and leucine into RNA and protein. Active cell replication was shown to be greatest during the first day of organ culture, and the number of cells that synthesized DNA also increased when deoxycholic acid was added to the culture medium. At later times, with morphological evidence of tissue viability, the synthesis of DNA, RNA, and protein within colonic epithelial cells decreased, and the total number of cells in the crypt columns declined. Findings indicate good maintenance of metabolic activities of colonic epithelial cells in short-term explant culture, and the utility of both cell-kinetic and morphological observations in assessing the status of colonic explants at early and late intervals.",
author = "M. Usugane and M. Fujita and M. Lipkin and R. Palmer and E. Friedman and Augenlicht, {Leonard H.}",
year = "1982",
language = "English (US)",
volume = "24",
pages = "225--233",
journal = "Digestion",
issn = "0012-2823",
publisher = "S. Karger AG",
number = "4",

}

TY - JOUR

T1 - Cell proliferation in explant cultures of human colon

AU - Usugane, M.

AU - Fujita, M.

AU - Lipkin, M.

AU - Palmer, R.

AU - Friedman, E.

AU - Augenlicht, Leonard H.

PY - 1982

Y1 - 1982

N2 - Biopsy specimens of human colonic mucosa taken from the rectosigmoid of 12 normal subjects were maintained in explant culture for 4 days. Histological, microautoradiographic and chemical measurements were carried out to evaluate cell replication, the effect of deoxycholic acid, and the incorporation of uridine and leucine into RNA and protein. Active cell replication was shown to be greatest during the first day of organ culture, and the number of cells that synthesized DNA also increased when deoxycholic acid was added to the culture medium. At later times, with morphological evidence of tissue viability, the synthesis of DNA, RNA, and protein within colonic epithelial cells decreased, and the total number of cells in the crypt columns declined. Findings indicate good maintenance of metabolic activities of colonic epithelial cells in short-term explant culture, and the utility of both cell-kinetic and morphological observations in assessing the status of colonic explants at early and late intervals.

AB - Biopsy specimens of human colonic mucosa taken from the rectosigmoid of 12 normal subjects were maintained in explant culture for 4 days. Histological, microautoradiographic and chemical measurements were carried out to evaluate cell replication, the effect of deoxycholic acid, and the incorporation of uridine and leucine into RNA and protein. Active cell replication was shown to be greatest during the first day of organ culture, and the number of cells that synthesized DNA also increased when deoxycholic acid was added to the culture medium. At later times, with morphological evidence of tissue viability, the synthesis of DNA, RNA, and protein within colonic epithelial cells decreased, and the total number of cells in the crypt columns declined. Findings indicate good maintenance of metabolic activities of colonic epithelial cells in short-term explant culture, and the utility of both cell-kinetic and morphological observations in assessing the status of colonic explants at early and late intervals.

UR - http://www.scopus.com/inward/record.url?scp=0020457580&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020457580&partnerID=8YFLogxK

M3 - Article

C2 - 7152147

AN - SCOPUS:0020457580

VL - 24

SP - 225

EP - 233

JO - Digestion

JF - Digestion

SN - 0012-2823

IS - 4

ER -