Cell-mediated immune response to epitopic MAP (multiple antigen peptide) construct of LcrV antigen of Yersinia pestis in murine model

Rupesh Kumar Shreewastav, Riyasat Ali, Jayaprakash Babu Uppada, D. N. Rao

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11 Scopus citations


Yersinia pestis is the causative agent of plague. Cellular immunity seems to play an important role in defense against this disease. The subunit vaccine based on V (Lcr V) antigen has been proved to be immunogenic in animals and in humans. The multiple antigen peptide (MAP), incorporating all the relevant B and T cell epitopes is highly immunogenic in mice through intranasal route of immunization in PLGA particles containing CpG-ODN as an immunoadjuvant inducing humoral and mucosal immune response. In the present study, cell-mediated immune response using same MAP was studied in murine model. Primary and memory T cell responses were studied in outbred and inbred mice immunized intranasally with MAP in the presence of two immunoadjuvants (Murabutide and CpG-ODN). All the three compartments (Spleen, Lamina propria and Peyer's patches) of the lymphoid system showed increased lymphoproliferative response. Highest lymphoproliferative response was observed especially with CpG-ODN. Cytokine profile in the culture supernatant showed highest Th1 and Th17 levels. FACS analysis showed expansion of both CD4+ and CD8+ T cells producing gamma-interferon, perforin and granzyme-B with major contribution from CD4+ T cells.



  • FACS
  • Granzyme-B
  • IFN-γ
  • LcrV
  • Multiple antigen peptide
  • PLGA
  • Perforin

ASJC Scopus subject areas

  • Immunology

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