Cell-mediated immune response to epitopic MAP (multiple antigen peptide) construct of LcrV antigen of Yersinia pestis in murine model

Rupesh Kumar Shreewastav, Riyasat Ali, Jayaprakash Babu Uppada, D. N. Rao

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Yersinia pestis is the causative agent of plague. Cellular immunity seems to play an important role in defense against this disease. The subunit vaccine based on V (Lcr V) antigen has been proved to be immunogenic in animals and in humans. The multiple antigen peptide (MAP), incorporating all the relevant B and T cell epitopes is highly immunogenic in mice through intranasal route of immunization in PLGA particles containing CpG-ODN as an immunoadjuvant inducing humoral and mucosal immune response. In the present study, cell-mediated immune response using same MAP was studied in murine model. Primary and memory T cell responses were studied in outbred and inbred mice immunized intranasally with MAP in the presence of two immunoadjuvants (Murabutide and CpG-ODN). All the three compartments (Spleen, Lamina propria and Peyer's patches) of the lymphoid system showed increased lymphoproliferative response. Highest lymphoproliferative response was observed especially with CpG-ODN. Cytokine profile in the culture supernatant showed highest Th1 and Th17 levels. FACS analysis showed expansion of both CD4+ and CD8+ T cells producing gamma-interferon, perforin and granzyme-B with major contribution from CD4+ T cells.

Original languageEnglish (US)
Pages (from-to)55-62
Number of pages8
JournalCellular Immunology
Volume278
Issue number1-2
DOIs
StatePublished - Jul 1 2012
Externally publishedYes

Fingerprint

Yersinia pestis
Antigens
Immunologic Adjuvants
Peptides
T-Lymphocytes
B-Lymphocyte Epitopes
Peyer's Patches
Mucosal Immunity
Subunit Vaccines
T-Lymphocyte Epitopes
Plague
Humoral Immunity
Cellular Immunity
Interferon-gamma
Immunization
Mucous Membrane
Spleen
Cytokines
difluprednate

Keywords

  • FACS
  • Granzyme-B
  • IFN-γ
  • LcrV
  • Multiple antigen peptide
  • Perforin
  • PLGA

ASJC Scopus subject areas

  • Immunology

Cite this

Cell-mediated immune response to epitopic MAP (multiple antigen peptide) construct of LcrV antigen of Yersinia pestis in murine model. / Shreewastav, Rupesh Kumar; Ali, Riyasat; Uppada, Jayaprakash Babu; Rao, D. N.

In: Cellular Immunology, Vol. 278, No. 1-2, 01.07.2012, p. 55-62.

Research output: Contribution to journalArticle

Shreewastav, Rupesh Kumar ; Ali, Riyasat ; Uppada, Jayaprakash Babu ; Rao, D. N. / Cell-mediated immune response to epitopic MAP (multiple antigen peptide) construct of LcrV antigen of Yersinia pestis in murine model. In: Cellular Immunology. 2012 ; Vol. 278, No. 1-2. pp. 55-62.
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