Cell lineage heterogeneity in blast crisis of chronic myeloid leukaemia

P. Bettelheim, D. Lutz, O. Majdic, Elisabeth M. Paietta, O. Haas, W. Linkesch, E. Neumann, K. Lechner, W. Knapp

Research output: Contribution to journalArticle

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Abstract

Blast cells from 45 patients with chronic myeloid leukaemia in blast crisis (CML-BC) were immunologically phenotyped with a panel of 26 monoclonal antibodies and studied for terminal deoxynucleotidyl transferase (TdT) content. Out of 45 blast-populations, 28 showed a myeloid, 14 a lymphoid, two a mixed and one an unclassifiable marker profile. In contrast to acute myeloid leukaemia (AML), we found frequent involvement of the thrombopoietic and erythropoietic systems in myeloid CML-BC. Furthermore, the marker profile on blast cells in myeloid CML-BC was different from that seen in AML. The blast cells in lymphoid blast crises of CML displayed the same lymphoid marker profile as those in acute lymphoblastic leukaemia. In three of 16 patients who were serially tested, we observed phenotypic changes in the blast cell populations. In one patient the blasts changed from lymphoid to myeloid type while remaining TdT-positive; in another case the blasts switched from granulomonocytic TdT-negative to granulomonocytic TdT-positive. In the third patient erythroid precursor cells appeared as the disease progressed. The results indicate the capacity of blast populations in CML-patients during blast crisis to differentiate along several pathways.

Original languageEnglish (US)
Pages (from-to)395-409
Number of pages15
JournalBritish Journal of Haematology
Volume59
Issue number3
StatePublished - 1985
Externally publishedYes

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Blast Crisis
Cell Lineage
Leukemia, Myelogenous, Chronic, BCR-ABL Positive
DNA Nucleotidylexotransferase
Acute Myeloid Leukemia
Population
Erythroid Precursor Cells
Myeloid Cells
Precursor Cell Lymphoblastic Leukemia-Lymphoma
Monoclonal Antibodies
Lymphocytes

ASJC Scopus subject areas

  • Hematology

Cite this

Bettelheim, P., Lutz, D., Majdic, O., Paietta, E. M., Haas, O., Linkesch, W., ... Knapp, W. (1985). Cell lineage heterogeneity in blast crisis of chronic myeloid leukaemia. British Journal of Haematology, 59(3), 395-409.

Cell lineage heterogeneity in blast crisis of chronic myeloid leukaemia. / Bettelheim, P.; Lutz, D.; Majdic, O.; Paietta, Elisabeth M.; Haas, O.; Linkesch, W.; Neumann, E.; Lechner, K.; Knapp, W.

In: British Journal of Haematology, Vol. 59, No. 3, 1985, p. 395-409.

Research output: Contribution to journalArticle

Bettelheim, P, Lutz, D, Majdic, O, Paietta, EM, Haas, O, Linkesch, W, Neumann, E, Lechner, K & Knapp, W 1985, 'Cell lineage heterogeneity in blast crisis of chronic myeloid leukaemia', British Journal of Haematology, vol. 59, no. 3, pp. 395-409.
Bettelheim P, Lutz D, Majdic O, Paietta EM, Haas O, Linkesch W et al. Cell lineage heterogeneity in blast crisis of chronic myeloid leukaemia. British Journal of Haematology. 1985;59(3):395-409.
Bettelheim, P. ; Lutz, D. ; Majdic, O. ; Paietta, Elisabeth M. ; Haas, O. ; Linkesch, W. ; Neumann, E. ; Lechner, K. ; Knapp, W. / Cell lineage heterogeneity in blast crisis of chronic myeloid leukaemia. In: British Journal of Haematology. 1985 ; Vol. 59, No. 3. pp. 395-409.
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