Cell internalization SELEX: In vitro selection for molecules that internalize into cells

Amy Yan, Matthew Levy

Research output: Chapter in Book/Report/Conference proceedingChapter

20 Scopus citations

Abstract

Aptamer technology allows for the selection of nucleic acids that can bind to and enter cells. By establishing conditions during the selection that eliminate cell-surface binders as well as non-internalizing RNAs, only extremely tightly bound aptamers or aptamers that have internalized are recovered. We describe a general scheme for selecting RNA molecules that are capable of internalizing into cells and discuss the factors that can affect a successful selection. Much like standard cell-surface selections, these types of selections should be possible independent of detailed knowledge of the cell surface.

Original languageEnglish (US)
Title of host publicationTherapeutic Applications of Ribozymes and Riboswitches
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages241-265
Number of pages25
ISBN (Print)9781627037297
DOIs
StatePublished - 2014

Publication series

NameMethods in Molecular Biology
Volume1103
ISSN (Print)1064-3745

Keywords

  • Aptamer
  • Cell internalization
  • In vitro selection
  • SELEX
  • Targeted delivery

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Yan, A., & Levy, M. (2014). Cell internalization SELEX: In vitro selection for molecules that internalize into cells. In Therapeutic Applications of Ribozymes and Riboswitches: Methods and Protocols (pp. 241-265). (Methods in Molecular Biology; Vol. 1103). Humana Press Inc.. https://doi.org/10.1007/978-1-62703-730-3_18