Cell-free translation of RNA isolated from aldosterone-treated toad bladder mucosal cells

Chia Ping H. Yang; Irene A. Skipski; Walter N. Scott

doi:10.1016/0005-2787(81)90139-8

Cell-free translation of RNA isolated from aldosterone-treated toad bladder mucosal cells

Chia Ping H. Yang, Irene A. Skipski, Walter N. Scott

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

The stimulation of sodium transport by aldosterone in target tissues requires the synthesis of both mRNA and proteins. Aldosterone-induced mRNA and proteins have been demonstrated in toad urinary bladder and rat kidney. We have isolated total RNA and poly(A)-containing RNA from hormone-treated and untreated toad bladder mucosal cells for translation in a rabbit reticulocyte lysate system. Aldosterone-induced proteins synthesized in this system have physical properties similar to those of aldosterone-induced proteins synthesized in the intact toad bladder.

Original language	English (US)
Pages (from-to)	86-93
Number of pages	8
Journal	BBA Section Nucleic Acids And Protein Synthesis
Volume	654
Issue number	1
DOIs	https://doi.org/10.1016/0005-2787(81)90139-8
State	Published - Jun 26 1981
Externally published	Yes

Keywords

(Toad bladder mucosal cell)
Aldosterone effect
Protein synthesis
RNA synthesis
Translation

ASJC Scopus subject areas

Medicine(all)

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10.1016/0005-2787(81)90139-8

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title = "Cell-free translation of RNA isolated from aldosterone-treated toad bladder mucosal cells",

abstract = "The stimulation of sodium transport by aldosterone in target tissues requires the synthesis of both mRNA and proteins. Aldosterone-induced mRNA and proteins have been demonstrated in toad urinary bladder and rat kidney. We have isolated total RNA and poly(A)-containing RNA from hormone-treated and untreated toad bladder mucosal cells for translation in a rabbit reticulocyte lysate system. Aldosterone-induced proteins synthesized in this system have physical properties similar to those of aldosterone-induced proteins synthesized in the intact toad bladder.",

keywords = "(Toad bladder mucosal cell), Aldosterone effect, Protein synthesis, RNA synthesis, Translation",

author = "Yang, {Chia Ping H.} and Skipski, {Irene A.} and Scott, {Walter N.}",

note = "Funding Information: This work was supported by the National Science Foundation (PCM 7923513).",

year = "1981",

month = jun,

day = "26",

doi = "10.1016/0005-2787(81)90139-8",

language = "English (US)",

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pages = "86--93",

journal = "BBA Section Nucleic Acids And Protein Synthesis",

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T1 - Cell-free translation of RNA isolated from aldosterone-treated toad bladder mucosal cells

AU - Yang, Chia Ping H.

AU - Skipski, Irene A.

AU - Scott, Walter N.

N1 - Funding Information: This work was supported by the National Science Foundation (PCM 7923513).

PY - 1981/6/26

Y1 - 1981/6/26

N2 - The stimulation of sodium transport by aldosterone in target tissues requires the synthesis of both mRNA and proteins. Aldosterone-induced mRNA and proteins have been demonstrated in toad urinary bladder and rat kidney. We have isolated total RNA and poly(A)-containing RNA from hormone-treated and untreated toad bladder mucosal cells for translation in a rabbit reticulocyte lysate system. Aldosterone-induced proteins synthesized in this system have physical properties similar to those of aldosterone-induced proteins synthesized in the intact toad bladder.

AB - The stimulation of sodium transport by aldosterone in target tissues requires the synthesis of both mRNA and proteins. Aldosterone-induced mRNA and proteins have been demonstrated in toad urinary bladder and rat kidney. We have isolated total RNA and poly(A)-containing RNA from hormone-treated and untreated toad bladder mucosal cells for translation in a rabbit reticulocyte lysate system. Aldosterone-induced proteins synthesized in this system have physical properties similar to those of aldosterone-induced proteins synthesized in the intact toad bladder.

KW - (Toad bladder mucosal cell)

KW - Aldosterone effect

KW - Protein synthesis

KW - RNA synthesis

KW - Translation

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VL - 654

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EP - 93

JO - BBA Section Nucleic Acids And Protein Synthesis

JF - BBA Section Nucleic Acids And Protein Synthesis

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ER -

Cell-free translation of RNA isolated from aldosterone-treated toad bladder mucosal cells

Abstract

Keywords

ASJC Scopus subject areas

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