Cell-free translation of RNA isolated from aldosterone-treated toad bladder mucosal cells

Chia Ping H. Yang, Irene A. Skipski, Walter N. Scott

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

The stimulation of sodium transport by aldosterone in target tissues requires the synthesis of both mRNA and proteins. Aldosterone-induced mRNA and proteins have been demonstrated in toad urinary bladder and rat kidney. We have isolated total RNA and poly(A)-containing RNA from hormone-treated and untreated toad bladder mucosal cells for translation in a rabbit reticulocyte lysate system. Aldosterone-induced proteins synthesized in this system have physical properties similar to those of aldosterone-induced proteins synthesized in the intact toad bladder.

Original languageEnglish (US)
Pages (from-to)86-93
Number of pages8
JournalBBA Section Nucleic Acids And Protein Synthesis
Volume654
Issue number1
DOIs
StatePublished - Jun 26 1981
Externally publishedYes

Keywords

  • (Toad bladder mucosal cell)
  • Aldosterone effect
  • Protein synthesis
  • RNA synthesis
  • Translation

ASJC Scopus subject areas

  • Medicine(all)

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