Cell-free fetal DNA in the cerebrospinal fluid of women during the peripartum period

Robert M. Angert, Erik S. LeShane, Ralph W. Yarnell, Kirby L. Johnson, Diana W. Bianchi

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Objective: The purpose of this study was to determine whether cell-free fetal DNA is detectable in the cerebrospinal fluid of women during pregnancy and after delivery. Study design: Cerebrospinal fluid was collected from 39 women who underwent an indicated spinal anesthesia procedure. Twenty-six samples were from women who carried at least 1 male fetus, and 13 samples were from women with only a female fetus. DNA was analyzed with the use of real-time polymerase chain reaction for DYS-1 (which represented male fetal DNA) and β-globin (which represented maternal and fetal DNA). Results: β-Globin DNA was detected in all cerebrospinal samples. DYS-1 gene sequences were detected in 4 cerebrospinal fluid samples from women who had male fetuses (2 samples were from women who underwent cesarean delivery of singleton pregnancies, 1 sample was from a triplet pregnancy, and 1 sample was from a woman after delivery). No male DNA was detected in the cerebrospinal fluid of women who carried female fetuses. Conclusion: Male fetal cells and/or cell-free fetal DNA is detectable in the cerebrospinal fluid of some pregnant women or some women after delivery.

Original languageEnglish (US)
Pages (from-to)1087-1090
Number of pages4
JournalAmerican Journal of Obstetrics and Gynecology
Volume190
Issue number4
DOIs
StatePublished - Apr 2004
Externally publishedYes

Fingerprint

Peripartum Period
Cerebrospinal Fluid
DNA
Fetus
Globins
Triplet Pregnancy
Pregnancy
Spinal Anesthesia
Real-Time Polymerase Chain Reaction
Pregnant Women
Mothers

Keywords

  • Cell-free fetal DNA
  • Cerebrospinal fluid
  • Fetomaternal trafficking
  • Spinal anesthesia

ASJC Scopus subject areas

  • Medicine(all)
  • Obstetrics and Gynecology

Cite this

Cell-free fetal DNA in the cerebrospinal fluid of women during the peripartum period. / Angert, Robert M.; LeShane, Erik S.; Yarnell, Ralph W.; Johnson, Kirby L.; Bianchi, Diana W.

In: American Journal of Obstetrics and Gynecology, Vol. 190, No. 4, 04.2004, p. 1087-1090.

Research output: Contribution to journalArticle

Angert, Robert M. ; LeShane, Erik S. ; Yarnell, Ralph W. ; Johnson, Kirby L. ; Bianchi, Diana W. / Cell-free fetal DNA in the cerebrospinal fluid of women during the peripartum period. In: American Journal of Obstetrics and Gynecology. 2004 ; Vol. 190, No. 4. pp. 1087-1090.
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