Cell cycle regulatory proteins in the liver in murine Trypanosoma cruzi infection

Boumediene Bouzahzah, Fnu Nagajyothi, Mahalia S. Desruisseaux, Mohan Krishnamachary, Stephen M. Factor, Alex W. Cohen, Michael P. Lisanti, Stefka B. Petkova, Richard G. Pestell, Murray Wittner, Shankar Mukherjee, Louis M. Weiss, Linda A. Jelicks, Chris Albanese, Herbert B. Tanowitz

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

The liver is an important target of Trypanosoma cruzi infection. Infection of CD-1 mice with T. cruzi (Brazil strain) resulted in parasitism of the liver, primarily in sinusoidal and Kupffer cells. Immunoblot analysis revealed activation of extra cellular signal-regulated kinase (ERK) during the acute and subacute period of infection, but p38 mitogen activated kinase (MAPK) and JNK were not activated. The activity of important cell cycle regulatory genes was also examined in the liver following infection. There was increased expression of cyclin D1, cyclin E and cyclin A as well as proliferating cell nuclear antigen (PCNA) at 45, 60 and 215 days post infection. In addition, the levels of the cyclin-dependent kinase inhibitors p27KIP1, p21WAF1 and the tumor suppressor p53 were increased in the livers obtained from infected mice. Quantitative PCR revealed increased abundance of mRNA for cyclins A, D1 and E. Interestingly, cyclin A and E are ordinarily not found in the adult liver. Thus infection caused a reversion to a fetal/neonatal phenotype. These data provide a molecular basis for cell proliferation in the liver following T. cruzi infection.

Original languageEnglish (US)
Pages (from-to)2396-2400
Number of pages5
JournalCell Cycle
Volume5
Issue number20
DOIs
StatePublished - Oct 15 2006

Keywords

  • Cell cycle regulatory proteins
  • Chagas' disease
  • Cyclin A
  • Cyclin D1
  • Cylin E
  • Liver
  • Mitogen-activated protein kinase
  • Trypanosoma cruzi

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

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    Bouzahzah, B., Nagajyothi, F., Desruisseaux, M. S., Krishnamachary, M., Factor, S. M., Cohen, A. W., Lisanti, M. P., Petkova, S. B., Pestell, R. G., Wittner, M., Mukherjee, S., Weiss, L. M., Jelicks, L. A., Albanese, C., & Tanowitz, H. B. (2006). Cell cycle regulatory proteins in the liver in murine Trypanosoma cruzi infection. Cell Cycle, 5(20), 2396-2400. https://doi.org/10.4161/cc.5.20.3380