Cell-by-cell scanning of whole mitochondrial genomes in aged human heart reveals a significant fraction of myocytes with clonally expanded deletions

Konstantin Khrapko, Natalya Bodyak, William G. Thilly, Nathalie J. Van Orsouw, Xiaomin Zhang, Hilary A. Coller, Thomas T. Peris, Melissa Upton, Jan Vijg, Jeanne Y. Wei

Research output: Contribution to journalArticle

146 Citations (Scopus)

Abstract

Quantitative information on the cell-to-cell distribution of all possible mitochondrial DNA (mtDNA) mutations in young and aged tissues is needed to assess the relevance of these mutations to the aging process. In the present study, we used PCR amplification of full-length mitochondrial genomes from single cells to scan human cardiomyocytes for all possible large deletions in mtDNA. Analysis of more than 350 individual cells that were derived from three middle-aged and four centenarian donors demonstrates that while most of the cells contain no deletions, in certain cardiomyocytes a significant portion of the mtDNA molecules carried one particular deletion. Different affected cells contained different deletions. Although similar numbers of cells were screened for each donor, these deletion-rich cells were found only in the hearts of old donors, where they occurred at a frequency of up to one in seven cells. These initial observations demonstrate the efficiency of the method and indicate that mitochondrial mutations have the potential to play an important role in human myocardial aging.

Original languageEnglish (US)
Pages (from-to)2434-2441
Number of pages8
JournalNucleic Acids Research
Volume27
Issue number11
StatePublished - Jun 1 1999
Externally publishedYes

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Mitochondrial Genome
Muscle Cells
Mitochondrial DNA
Tissue Donors
Cardiac Myocytes
Mutation
Cell Count
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Genetics

Cite this

Khrapko, K., Bodyak, N., Thilly, W. G., Van Orsouw, N. J., Zhang, X., Coller, H. A., ... Wei, J. Y. (1999). Cell-by-cell scanning of whole mitochondrial genomes in aged human heart reveals a significant fraction of myocytes with clonally expanded deletions. Nucleic Acids Research, 27(11), 2434-2441.

Cell-by-cell scanning of whole mitochondrial genomes in aged human heart reveals a significant fraction of myocytes with clonally expanded deletions. / Khrapko, Konstantin; Bodyak, Natalya; Thilly, William G.; Van Orsouw, Nathalie J.; Zhang, Xiaomin; Coller, Hilary A.; Peris, Thomas T.; Upton, Melissa; Vijg, Jan; Wei, Jeanne Y.

In: Nucleic Acids Research, Vol. 27, No. 11, 01.06.1999, p. 2434-2441.

Research output: Contribution to journalArticle

Khrapko, K, Bodyak, N, Thilly, WG, Van Orsouw, NJ, Zhang, X, Coller, HA, Peris, TT, Upton, M, Vijg, J & Wei, JY 1999, 'Cell-by-cell scanning of whole mitochondrial genomes in aged human heart reveals a significant fraction of myocytes with clonally expanded deletions', Nucleic Acids Research, vol. 27, no. 11, pp. 2434-2441.
Khrapko, Konstantin ; Bodyak, Natalya ; Thilly, William G. ; Van Orsouw, Nathalie J. ; Zhang, Xiaomin ; Coller, Hilary A. ; Peris, Thomas T. ; Upton, Melissa ; Vijg, Jan ; Wei, Jeanne Y. / Cell-by-cell scanning of whole mitochondrial genomes in aged human heart reveals a significant fraction of myocytes with clonally expanded deletions. In: Nucleic Acids Research. 1999 ; Vol. 27, No. 11. pp. 2434-2441.
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