Cell behavior and actomyosin organization in Dictyostelium during substrate exploration

Y. Fukui, J. Murray, John W. Murray, D. R. Soll

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The behavior of individual Dictyostellum amebae was quantitatively analyzed with the computer-assisted ″Dynamic Morphology System″ (Soll, Voss, Varnum-Finney and Wessels (1988) J. Cell. Biochem., 37:177-192.). The same amoebae were then fixed and analyzed for filamentous (F-) actin and myosin (myosin-II, or ″coventional″ myosin) by fluorescence microscopy using the ″agar overlay method″ (Yumura, and Fukui (1985) Nature, 314:194-196.). This procedure provides a novel description of the behavior and morphometric changes preceding the static analysis of cytoskeletal organization in the same cell. It is demonstrated that when translocating cells make contact with an etched-smooth glass interface, 14% cross the interface, 20% either reverse direction or migrate along the interface, and the remaining 45% stay at the site. Cells contacting the interface from the smooth or etched side show equivalent behavioral responses. Upon contact with the interface, they project numerous lamellipodia and pseudopodia. While the lamellipodial projections exhibit cycles of spreading and retraction, the pseudopodia show lateral scanning motion, analogous to ″substrate exploration″ in fibroblasts (Albrecht-Buehler (1976) J. Cell Biol., 69: 275-286.). F-actin is localized in the lamellipodia and pseudopodia of amoebae contacting the interface. There is also discernable cortical F-actin while conventional myosin appears to be excluded from the cortex and dispersed throughout the cytoplasm. The myosin displays a transient filamentous lattice at the base of newly forming lamellipodia. The ultrastructural study suggests that the new lamellipodia are formed on the dorsal surface and subsequently make contact with the substrate, indicating the dorsoventral sequence of polarity of the motile/sensory cellular organs. The present study demonstrates substrate exploration in Dictyostelium amoebae, and suggests its coupling to dynamic reorganization of the actomyosin cytoskeleton. The possible role of single-headed small myosin(s) (myosin-I or mini-myosin) is discussed.

Original languageEnglish (US)
Pages (from-to)289-301
Number of pages13
JournalCell Structure and Function
Volume16
Issue number4
StatePublished - 1991
Externally publishedYes

Fingerprint

Actomyosin
Pseudopodia
Dictyostelium
Myosins
Amoeba
Actins
Myosin Type I
Myosin Type II
Cytoskeleton
Fluorescence Microscopy
Agar
Glass
Cytoplasm
Fibroblasts

Keywords

  • Etched covership
  • Immunofluorescence
  • Lamellipodium Mechanoreception
  • Video motion analysis

ASJC Scopus subject areas

  • Physiology
  • Structural Biology
  • Cell Biology

Cite this

Cell behavior and actomyosin organization in Dictyostelium during substrate exploration. / Fukui, Y.; Murray, J.; Murray, John W.; Soll, D. R.

In: Cell Structure and Function, Vol. 16, No. 4, 1991, p. 289-301.

Research output: Contribution to journalArticle

@article{23d668d657af4f7fbec731ddfb4e36f1,
title = "Cell behavior and actomyosin organization in Dictyostelium during substrate exploration",
abstract = "The behavior of individual Dictyostellum amebae was quantitatively analyzed with the computer-assisted ″Dynamic Morphology System″ (Soll, Voss, Varnum-Finney and Wessels (1988) J. Cell. Biochem., 37:177-192.). The same amoebae were then fixed and analyzed for filamentous (F-) actin and myosin (myosin-II, or ″coventional″ myosin) by fluorescence microscopy using the ″agar overlay method″ (Yumura, and Fukui (1985) Nature, 314:194-196.). This procedure provides a novel description of the behavior and morphometric changes preceding the static analysis of cytoskeletal organization in the same cell. It is demonstrated that when translocating cells make contact with an etched-smooth glass interface, 14{\%} cross the interface, 20{\%} either reverse direction or migrate along the interface, and the remaining 45{\%} stay at the site. Cells contacting the interface from the smooth or etched side show equivalent behavioral responses. Upon contact with the interface, they project numerous lamellipodia and pseudopodia. While the lamellipodial projections exhibit cycles of spreading and retraction, the pseudopodia show lateral scanning motion, analogous to ″substrate exploration″ in fibroblasts (Albrecht-Buehler (1976) J. Cell Biol., 69: 275-286.). F-actin is localized in the lamellipodia and pseudopodia of amoebae contacting the interface. There is also discernable cortical F-actin while conventional myosin appears to be excluded from the cortex and dispersed throughout the cytoplasm. The myosin displays a transient filamentous lattice at the base of newly forming lamellipodia. The ultrastructural study suggests that the new lamellipodia are formed on the dorsal surface and subsequently make contact with the substrate, indicating the dorsoventral sequence of polarity of the motile/sensory cellular organs. The present study demonstrates substrate exploration in Dictyostelium amoebae, and suggests its coupling to dynamic reorganization of the actomyosin cytoskeleton. The possible role of single-headed small myosin(s) (myosin-I or mini-myosin) is discussed.",
keywords = "Etched covership, Immunofluorescence, Lamellipodium Mechanoreception, Video motion analysis",
author = "Y. Fukui and J. Murray and Murray, {John W.} and Soll, {D. R.}",
year = "1991",
language = "English (US)",
volume = "16",
pages = "289--301",
journal = "Cell Structure and Function",
issn = "0386-7196",
publisher = "Japan Society for Cell Biology",
number = "4",

}

TY - JOUR

T1 - Cell behavior and actomyosin organization in Dictyostelium during substrate exploration

AU - Fukui, Y.

AU - Murray, J.

AU - Murray, John W.

AU - Soll, D. R.

PY - 1991

Y1 - 1991

N2 - The behavior of individual Dictyostellum amebae was quantitatively analyzed with the computer-assisted ″Dynamic Morphology System″ (Soll, Voss, Varnum-Finney and Wessels (1988) J. Cell. Biochem., 37:177-192.). The same amoebae were then fixed and analyzed for filamentous (F-) actin and myosin (myosin-II, or ″coventional″ myosin) by fluorescence microscopy using the ″agar overlay method″ (Yumura, and Fukui (1985) Nature, 314:194-196.). This procedure provides a novel description of the behavior and morphometric changes preceding the static analysis of cytoskeletal organization in the same cell. It is demonstrated that when translocating cells make contact with an etched-smooth glass interface, 14% cross the interface, 20% either reverse direction or migrate along the interface, and the remaining 45% stay at the site. Cells contacting the interface from the smooth or etched side show equivalent behavioral responses. Upon contact with the interface, they project numerous lamellipodia and pseudopodia. While the lamellipodial projections exhibit cycles of spreading and retraction, the pseudopodia show lateral scanning motion, analogous to ″substrate exploration″ in fibroblasts (Albrecht-Buehler (1976) J. Cell Biol., 69: 275-286.). F-actin is localized in the lamellipodia and pseudopodia of amoebae contacting the interface. There is also discernable cortical F-actin while conventional myosin appears to be excluded from the cortex and dispersed throughout the cytoplasm. The myosin displays a transient filamentous lattice at the base of newly forming lamellipodia. The ultrastructural study suggests that the new lamellipodia are formed on the dorsal surface and subsequently make contact with the substrate, indicating the dorsoventral sequence of polarity of the motile/sensory cellular organs. The present study demonstrates substrate exploration in Dictyostelium amoebae, and suggests its coupling to dynamic reorganization of the actomyosin cytoskeleton. The possible role of single-headed small myosin(s) (myosin-I or mini-myosin) is discussed.

AB - The behavior of individual Dictyostellum amebae was quantitatively analyzed with the computer-assisted ″Dynamic Morphology System″ (Soll, Voss, Varnum-Finney and Wessels (1988) J. Cell. Biochem., 37:177-192.). The same amoebae were then fixed and analyzed for filamentous (F-) actin and myosin (myosin-II, or ″coventional″ myosin) by fluorescence microscopy using the ″agar overlay method″ (Yumura, and Fukui (1985) Nature, 314:194-196.). This procedure provides a novel description of the behavior and morphometric changes preceding the static analysis of cytoskeletal organization in the same cell. It is demonstrated that when translocating cells make contact with an etched-smooth glass interface, 14% cross the interface, 20% either reverse direction or migrate along the interface, and the remaining 45% stay at the site. Cells contacting the interface from the smooth or etched side show equivalent behavioral responses. Upon contact with the interface, they project numerous lamellipodia and pseudopodia. While the lamellipodial projections exhibit cycles of spreading and retraction, the pseudopodia show lateral scanning motion, analogous to ″substrate exploration″ in fibroblasts (Albrecht-Buehler (1976) J. Cell Biol., 69: 275-286.). F-actin is localized in the lamellipodia and pseudopodia of amoebae contacting the interface. There is also discernable cortical F-actin while conventional myosin appears to be excluded from the cortex and dispersed throughout the cytoplasm. The myosin displays a transient filamentous lattice at the base of newly forming lamellipodia. The ultrastructural study suggests that the new lamellipodia are formed on the dorsal surface and subsequently make contact with the substrate, indicating the dorsoventral sequence of polarity of the motile/sensory cellular organs. The present study demonstrates substrate exploration in Dictyostelium amoebae, and suggests its coupling to dynamic reorganization of the actomyosin cytoskeleton. The possible role of single-headed small myosin(s) (myosin-I or mini-myosin) is discussed.

KW - Etched covership

KW - Immunofluorescence

KW - Lamellipodium Mechanoreception

KW - Video motion analysis

UR - http://www.scopus.com/inward/record.url?scp=0025998893&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025998893&partnerID=8YFLogxK

M3 - Article

C2 - 1782667

AN - SCOPUS:0025998893

VL - 16

SP - 289

EP - 301

JO - Cell Structure and Function

JF - Cell Structure and Function

SN - 0386-7196

IS - 4

ER -