Cell autonomous roles for AP-2α in lens vesicle separation and maintenance of the lens epithelial cell phenotype

Giuseppe F. Pontoriero, Paula Deschamps, Ruth Ashery-Padan, Ryan Wong, Ying Yang, Jiri Zavadil, Ales Cvekl, Shelley Sullivan, Trevor Williams, Judith A. West-Mays

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

In this study, we have created a conditional deletion of AP-2α in the developing mouse lens (Le-AP-2α mutants) to determine the cell-autonomous requirement(s) for AP-2α in lens development. Embryonic and adult Le-AP-2α mutants exhibited defects confined to lens placode derivatives, including a persistent adhesion of the lens to the overlying corneal epithelium (or lens stalk). Expression of known regulators of lens vesicle separation, including Pax6, Pitx3, and Foxe3 was observed in the Le-AP-2α mutant lens demonstrating that these genes do not lie directly downstream of AP-2α. Unlike germ-line mutants, Le-AP-2α mutants did not exhibit defects in the optic cup, further defining the tissue specific role(s) for AP-2α in eye development. Finally, comparative microarray analysis of lenses from the Le-AP-2α mutants vs. wildtype littermates revealed differential expression of 415 mRNAs, including reduced expression of genes important for maintaining the lens epithelial cell phenotype, such as E-cadherin.

Original languageEnglish (US)
Pages (from-to)602-617
Number of pages16
JournalDevelopmental Dynamics
Volume237
Issue number3
DOIs
StatePublished - Mar 2008

Fingerprint

Lenses
Epithelial Cells
Maintenance
Phenotype
Corneal Epithelium
Cadherins
Microarray Analysis
Germ Cells
Gene Expression
Messenger RNA
Genes

Keywords

  • Activating protein-2alpha
  • E-cadherin, epidermal growth factor receptor
  • Lens development

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

Cite this

Pontoriero, G. F., Deschamps, P., Ashery-Padan, R., Wong, R., Yang, Y., Zavadil, J., ... West-Mays, J. A. (2008). Cell autonomous roles for AP-2α in lens vesicle separation and maintenance of the lens epithelial cell phenotype. Developmental Dynamics, 237(3), 602-617. https://doi.org/10.1002/dvdy.21445

Cell autonomous roles for AP-2α in lens vesicle separation and maintenance of the lens epithelial cell phenotype. / Pontoriero, Giuseppe F.; Deschamps, Paula; Ashery-Padan, Ruth; Wong, Ryan; Yang, Ying; Zavadil, Jiri; Cvekl, Ales; Sullivan, Shelley; Williams, Trevor; West-Mays, Judith A.

In: Developmental Dynamics, Vol. 237, No. 3, 03.2008, p. 602-617.

Research output: Contribution to journalArticle

Pontoriero, GF, Deschamps, P, Ashery-Padan, R, Wong, R, Yang, Y, Zavadil, J, Cvekl, A, Sullivan, S, Williams, T & West-Mays, JA 2008, 'Cell autonomous roles for AP-2α in lens vesicle separation and maintenance of the lens epithelial cell phenotype', Developmental Dynamics, vol. 237, no. 3, pp. 602-617. https://doi.org/10.1002/dvdy.21445
Pontoriero, Giuseppe F. ; Deschamps, Paula ; Ashery-Padan, Ruth ; Wong, Ryan ; Yang, Ying ; Zavadil, Jiri ; Cvekl, Ales ; Sullivan, Shelley ; Williams, Trevor ; West-Mays, Judith A. / Cell autonomous roles for AP-2α in lens vesicle separation and maintenance of the lens epithelial cell phenotype. In: Developmental Dynamics. 2008 ; Vol. 237, No. 3. pp. 602-617.
@article{5e6f6ac200e84f3aac3f6f0eb24a56f9,
title = "Cell autonomous roles for AP-2α in lens vesicle separation and maintenance of the lens epithelial cell phenotype",
abstract = "In this study, we have created a conditional deletion of AP-2α in the developing mouse lens (Le-AP-2α mutants) to determine the cell-autonomous requirement(s) for AP-2α in lens development. Embryonic and adult Le-AP-2α mutants exhibited defects confined to lens placode derivatives, including a persistent adhesion of the lens to the overlying corneal epithelium (or lens stalk). Expression of known regulators of lens vesicle separation, including Pax6, Pitx3, and Foxe3 was observed in the Le-AP-2α mutant lens demonstrating that these genes do not lie directly downstream of AP-2α. Unlike germ-line mutants, Le-AP-2α mutants did not exhibit defects in the optic cup, further defining the tissue specific role(s) for AP-2α in eye development. Finally, comparative microarray analysis of lenses from the Le-AP-2α mutants vs. wildtype littermates revealed differential expression of 415 mRNAs, including reduced expression of genes important for maintaining the lens epithelial cell phenotype, such as E-cadherin.",
keywords = "Activating protein-2alpha, E-cadherin, epidermal growth factor receptor, Lens development",
author = "Pontoriero, {Giuseppe F.} and Paula Deschamps and Ruth Ashery-Padan and Ryan Wong and Ying Yang and Jiri Zavadil and Ales Cvekl and Shelley Sullivan and Trevor Williams and West-Mays, {Judith A.}",
year = "2008",
month = "3",
doi = "10.1002/dvdy.21445",
language = "English (US)",
volume = "237",
pages = "602--617",
journal = "Developmental Dynamics",
issn = "1058-8388",
publisher = "Wiley-Liss Inc.",
number = "3",

}

TY - JOUR

T1 - Cell autonomous roles for AP-2α in lens vesicle separation and maintenance of the lens epithelial cell phenotype

AU - Pontoriero, Giuseppe F.

AU - Deschamps, Paula

AU - Ashery-Padan, Ruth

AU - Wong, Ryan

AU - Yang, Ying

AU - Zavadil, Jiri

AU - Cvekl, Ales

AU - Sullivan, Shelley

AU - Williams, Trevor

AU - West-Mays, Judith A.

PY - 2008/3

Y1 - 2008/3

N2 - In this study, we have created a conditional deletion of AP-2α in the developing mouse lens (Le-AP-2α mutants) to determine the cell-autonomous requirement(s) for AP-2α in lens development. Embryonic and adult Le-AP-2α mutants exhibited defects confined to lens placode derivatives, including a persistent adhesion of the lens to the overlying corneal epithelium (or lens stalk). Expression of known regulators of lens vesicle separation, including Pax6, Pitx3, and Foxe3 was observed in the Le-AP-2α mutant lens demonstrating that these genes do not lie directly downstream of AP-2α. Unlike germ-line mutants, Le-AP-2α mutants did not exhibit defects in the optic cup, further defining the tissue specific role(s) for AP-2α in eye development. Finally, comparative microarray analysis of lenses from the Le-AP-2α mutants vs. wildtype littermates revealed differential expression of 415 mRNAs, including reduced expression of genes important for maintaining the lens epithelial cell phenotype, such as E-cadherin.

AB - In this study, we have created a conditional deletion of AP-2α in the developing mouse lens (Le-AP-2α mutants) to determine the cell-autonomous requirement(s) for AP-2α in lens development. Embryonic and adult Le-AP-2α mutants exhibited defects confined to lens placode derivatives, including a persistent adhesion of the lens to the overlying corneal epithelium (or lens stalk). Expression of known regulators of lens vesicle separation, including Pax6, Pitx3, and Foxe3 was observed in the Le-AP-2α mutant lens demonstrating that these genes do not lie directly downstream of AP-2α. Unlike germ-line mutants, Le-AP-2α mutants did not exhibit defects in the optic cup, further defining the tissue specific role(s) for AP-2α in eye development. Finally, comparative microarray analysis of lenses from the Le-AP-2α mutants vs. wildtype littermates revealed differential expression of 415 mRNAs, including reduced expression of genes important for maintaining the lens epithelial cell phenotype, such as E-cadherin.

KW - Activating protein-2alpha

KW - E-cadherin, epidermal growth factor receptor

KW - Lens development

UR - http://www.scopus.com/inward/record.url?scp=40849094420&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=40849094420&partnerID=8YFLogxK

U2 - 10.1002/dvdy.21445

DO - 10.1002/dvdy.21445

M3 - Article

C2 - 18224708

AN - SCOPUS:40849094420

VL - 237

SP - 602

EP - 617

JO - Developmental Dynamics

JF - Developmental Dynamics

SN - 1058-8388

IS - 3

ER -