CEF1/CDC5 alleles modulate transitions between catalytic conformations of the spliceosome

Charles C. Query, Maria M. Konarska

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Conformational change within the spliceosome is required between the first and second catalytic steps of pre-mRNA splicing. A prior genetic screen for suppressors of an intron mutant that stalls between the two steps yielded both prp8 and non-prp8 alleles that suppressed second-step splicing defects. We have now identified the strongest non-prp8 suppressors as alleles of the NTC (Prp19 complex) component, CEF1. These cef1 alleles generally suppress second-step defects caused by a variety of intron mutations, mutations in U6 snRNA, or deletion of the second-step protein factor Prp17, and they can activate alternative 39 splice sites. Genetic and functional interactions between cef1 and prp8 alleles suggest that they modulate the same event(s) in the first-to-second-step transition, most likely by stabilization of the second-step spliceosome; in contrast, alleles of U6 snRNA that also alter this transition modulate a distinct event, most likely by stabilization of the first-step spliceosome. These results implicate a myb-like domain of Cef1/CDC5 in interactions that modulate conformational states of the spliceosome and suggest that alteration of these events affects splice site use, resulting in alternative splicing-like patterns in yeast. Published by Cold Spring Harbor Laboratory Press.

Original languageEnglish (US)
Pages (from-to)1001-1013
Number of pages13
JournalRNA
Volume18
Issue number5
DOIs
StatePublished - May 2012

Fingerprint

Spliceosomes
Alleles
Introns
RNA Splice Sites
Mutation
RNA Precursors
Alternative Splicing
Yeasts
Proteins

Keywords

  • Cef1
  • Fidelity
  • Intron mutations
  • NTC
  • Prp22
  • Prp8
  • U6 snRNA

ASJC Scopus subject areas

  • Molecular Biology

Cite this

CEF1/CDC5 alleles modulate transitions between catalytic conformations of the spliceosome. / Query, Charles C.; Konarska, Maria M.

In: RNA, Vol. 18, No. 5, 05.2012, p. 1001-1013.

Research output: Contribution to journalArticle

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