CDNA immunization of mice with human thyroglobulin generates both humoral and T cell responses: A novel model of thyroid autoimmunity

Eric M. Jacobson, Erlinda Concepcion, Kenneth Ho, Peter Kopp, Jussara Vono Toniolo, Yaron Tomer

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Thyroglobulin (Tg) represents one of the largest known self-antigens involved in autoimmunity. Numerous studies have implicated it in triggering and perpetuating the autoimmune response in autoimmune thyroid diseases (AITD). Indeed, traditional models of autoimmune thyroid disease, experimental autoimmune thyroiditis (EAT), are generated by immunizing mice with thyroglobulin protein in conjunction with an adjuvant, or by high repeated doses of Tg alone, without adjuvant. These extant models are limited in their experimental flexibility, i.e. the ability to make modifications to the Tg used in immunizations. In this study, we have immunized mice with a plasmid cDNA encoding the full-length human Tg (hTG) protein, in order to generate a model of Hashimoto's thyroiditis which is closer to the human disease and does not require adjuvants to breakdown tolerance. Human thyroglobulin cDNA was injected and subsequently electroporated into skeletal muscle using a square wave generator. Following hTg cDNA immunizations, the mice developed both B and T cell responses to Tg, albeit with no evidence of lymphocytic infiltration of the thyroid. This novel model will afford investigators the means to test various hypotheses which were unavailable with the previous EAT models, specifically the effects of hTg sequence variations on the induction of thyroiditis.

Original languageEnglish (US)
Article numbere19200
JournalPLoS One
Volume6
Issue number4
DOIs
StatePublished - 2011
Externally publishedYes

Fingerprint

thyroglobulin
Immunization
autoimmunity
T-cells
Thyroglobulin
Autoimmunity
immunization
Thyroid Gland
T-lymphocytes
T-Lymphocytes
mice
thyroid diseases
adjuvants
Autoimmune Thyroiditis
Complementary DNA
Thyroid Diseases
Autoimmune Diseases
Square wave generators
Thyroiditis
Hashimoto Disease

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

CDNA immunization of mice with human thyroglobulin generates both humoral and T cell responses : A novel model of thyroid autoimmunity. / Jacobson, Eric M.; Concepcion, Erlinda; Ho, Kenneth; Kopp, Peter; Vono Toniolo, Jussara; Tomer, Yaron.

In: PLoS One, Vol. 6, No. 4, e19200, 2011.

Research output: Contribution to journalArticle

Jacobson, Eric M. ; Concepcion, Erlinda ; Ho, Kenneth ; Kopp, Peter ; Vono Toniolo, Jussara ; Tomer, Yaron. / CDNA immunization of mice with human thyroglobulin generates both humoral and T cell responses : A novel model of thyroid autoimmunity. In: PLoS One. 2011 ; Vol. 6, No. 4.
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